UBR7 Polyclonal antibody

UBR7 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 16903-1-AP

Synonyms

C14orf130, N recognin 7, UBR7

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Validation Data Gallery

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  WB analysis using 16903-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16903-1-AP (UBR7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16903-1-AP targets UBR7 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UBR7 fusion protein Ag10445
• Full Name: ubiquitin protein ligase E3 component n-recognin 7 (putative)
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 48-60 kDa
• GenBank Accession Number: BC015046
• Gene Symbol: UBR7
• Gene ID (NCBI): 55148
• RRID: AB_2256707
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UBR7 (C14orf130), is a 425-amino-acid putative E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. The N-end rule pathway is a ubiquitin-dependent system where E3 ligases called N-recognins, including UBR1, UBR2 and UBR7 (N-recognin-7), leading to target protein ubiquitination and subsequent degradation (PMID: 19008229). UBR7 contains a PHD-type zinc finger and a UBR-type zinc finger domain (PMID: 14702039), and probably undergoes acetylation (PMID: 19413330). Till date its precise function is not well known. Proteintech's UBR7 antibody 16903-1-AP is able to detect UBR7 with a theoretical MW of 48 kDa.