Tested Applications
| Positive WB detected in | HeLa cells, human liver tissue, mouse cerebellum tissue, human brain tissue, human heart tissue, HepG2 cells, Ramos cells, SH-SY5Y cells, mouse liver tissue, rat liver tissue |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:30000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16135-1-AP targets UBXN1 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9146 Product name: Recombinant human UBXN1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-297 aa of BC000902 Sequence: MAELTALESLIEMGFPRGRAEKALALTGNQGIEAAMDWLMEHEDDPDVDEPLETPLGHILGREPTSSEQGGLEGSGSAAGEGKPALSEEERQEQTKRMLELVAQKQREREEREEREALERERQRRRQGQELSAARQRLQEDEMRRAAEERRREKAEELAARQRVREKIERDKAERAKKYGGSVGSQPPPVAPEPGPVPSSPSQEPPTKREYDQCRIQVRLPDGTSLTQTFRAREQLAAVRLYVELHRGEELGGGQDPVQLLSGFPRRAFSEADMERPLQELGLVPSAVLIVAKKCPS Predict reactive species |
| Full Name | UBX domain protein 1 |
| Calculated Molecular Weight | 33 kDa |
| Observed Molecular Weight | 40-45 kDa |
| GenBank Accession Number | BC000902 |
| Gene Symbol | UBXN1 |
| Gene ID (NCBI) | 51035 |
| RRID | AB_2210480 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q04323 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for UBXN1 antibody 16135-1-AP | Download protocol |
| WB protocol for UBXN1 antibody 16135-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-UBXN1 antibody (Cat# 16135-1-AP) has 13 citations across journals including Nature Communications, EMBO Journal, Cancer Letters, Cell Reports, EMBO Reports, Science China Life Sciences, Frontiers in Immunology, Journal of Cell Science, Molecular Cell Biology, Scientific Reports, Frontiers in Molecular Neuroscience, Veterinary Research, and Research Square. Research fields span cancer biology, viral infection, neurodevelopmental disorders, ER proteostasis, ubiquitin-mediated protein degradation, and innate immunity.
| Species | Application | Title |
|---|---|---|
Nat Commun The p97-UBXD8 complex regulates ER-Mitochondria contact sites by altering membrane lipid saturation and composition | ||
Cancer Lett The CRISPR/Cas9 system targeting EGFR exon 17 abrogates NF-κB activation via epigenetic modulation of UBXN1 in EGFRwt/vIII glioma cells. | ||
Sci China Life Sci SUB1 promotes colorectal cancer metastasis by activating NF-κB signaling via UBR5-mediated ubiquitination of UBXN1 | ||
EMBO J A weakened interface in the P182L variant of HSP27 associated with severe Charcot-Marie-Tooth neuropathy causes aberrant binding to interacting proteins. | ||
Front Immunol PTRF/Cavin-1 as a Novel RNA-Binding Protein Expedites the NF-κB/PD-L1 Axis by Stabilizing lncRNA NEAT1, Contributing to Tumorigenesis and Immune Evasion in Glioblastoma. |
