UBXN11 Polyclonal antibody

UBXN11 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 13109-1-AP

Synonyms

COA 1, PP2243, SOC, SOCI, Socius, UBX domain protein 11, UBXD5, UBXN11

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Validation Data Gallery

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  WB analysis of PC-3 using 13109-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13109-1-AP (UBXN11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 13109-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13109-1-AP (UBXN11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 13109-1-AP

  IP result of anti-UBXN11 (IP:13109-1-AP, 4ug; Detection:13109-1-AP 1:500) with mouse testis tissue lysate 4000ug.

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  IHC staining of human testis using 13109-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13109-1-AP (UBXN11 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 13109-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13109-1-AP (UBXN11 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human lung cancer using 13109-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13109-1-AP (UBXN11 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 13109-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13109-1-AP (UBXN11 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 13109-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 13109-1-AP (UBXN11 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 13109-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 13109-1-AP (UBXN11 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 13109-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13109-1-AP (UBXN11 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 13109-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13109-1-AP (UBXN11 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: PC-3 cells, mouse testis tissue
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human testis tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13109-1-AP targets UBXN11 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UBXN11 fusion protein Ag3772
• Full Name: UBX domain protein 11
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 48-54 kDa
• GenBank Accession Number: BC038106
• Gene Symbol: UBXN11
• Gene ID (NCBI): 91544
• RRID: AB_2288037
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
		J Cell Sci; The p97-UBXN1 complex regulates aggresome formation.; Authors - Sirisha Mukkavalli