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Various lysates were subjected to SDS PAGE followed by western blot with RMX00043 (UCH-L1/PGP9.5 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Various lysates were subjected to SDS PAGE followed by western blot with RMX00043 (UCH-L1/PGP9.5 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.
IP experiment of SH-SY5Y using RMX00043
IP result of anti-UCH-L1/PGP9.5 (IP:RMX00043, 4ug; Detection:RMX00043 1:50000) with SH-SY5Y cells lysate 1480 ug.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using RMX00043 (UCH-L1/PGP9.5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of A549 using RMX00043
Immunofluorescent analysis of (4% PFA) fixed A549 cells using UCH-L1/PGP9.5 antibody (RMX00043) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Immunofluorescent analysis of (4% PFA) fixed A549 cells using UCH-L1/PGP9.5 antibody (RMX00043) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
rat brain tissue, SH-SY5Y cells, Neuro-2a cells, mouse brain tissue
Positive IP detected in
SH-SY5Y cells
Positive IHC detected in
mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
A549 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:20000-1:100000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:200-1:800
Immunofluorescence (IF)/ICC
IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
RMX00043 targets UCH-L1/PGP9.5 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
UCHL1(Ubiquitin carboxyl-terminal hydrolase isozyme L1) is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of UCHL1 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. It is present in all neurons(PMID: 6343558). This protein is present in brain at concentrations at least 50 times greater than in other organs and is a major protein component of neuronal cytoplasm (PMID:7217993). And UCHL1 is a Parkinson's disease susceptibility gene (PMID:15048890).
This validation data has been generated in-house and reviewed by Proteintech scientists.
Find out more on our antibody validation policy
WB Figures
WB analysis using RMX00043
Various lysates were subjected to SDS PAGE followed by western blot with RMX00043 (UCH-L1/PGP9.5 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of mouse brain using RMX00043
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using RMX00043 (UCH-L1/PGP9.5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP Figures
IP experiment of SH-SY5Y using RMX00043
IP result of anti-UCH-L1/PGP9.5 (IP:RMX00043, 4ug; Detection:RMX00043 1:50000) with SH-SY5Y cells lysate 1480 ug.
IF/ICC Figures
IF Staining of A549 using RMX00043
Immunofluorescent analysis of (4% PFA) fixed A549 cells using UCH-L1/PGP9.5 antibody (RMX00043) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
UCH-L1/PGP9.5 Multi-Recombinant antibody
Catalog Number
RMX00043
Citations
-
Dilutions
WB : 1:20000-1:100000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:200-1:800 IF/ICC : 1:50-1:500
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
