UCHL3 Recombinant antibody, PBS Only

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  WB analysis using 84542-6-RR (same clone as 84542-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84542-6-RR (UCHL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84542-6-PBS in a different storage buffer formulation.

• 

  WB analysis using 84542-6-RR (same clone as 84542-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84542-6-RR (UCHL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84542-6-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 84542-6-RR (same clone as 84542-6-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84542-6-RR (UCHL3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84542-6-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 84542-6-RR

  Biolayer interferometry (BLl) kinetic assays of 84542-6-RR against Human UCHL3 were performed. The affinity constant is 2.45 nM.

UCHL3 Recombinant Antibody for WB, IHC, Indirect ELISA

Cat No. 84542-6-PBS

Clone No.242015A10

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

UCH-L3, UCH L3, Ubiquitin thioesterase L3, Ubiquitin carboxyl terminal hydrolase isozyme L3, EC:3.4.19.12

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Product Information

84542-6-PBS targets UCHL3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: UCHL3 fusion protein Ag3053
• Full Name: ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase)
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 26 kDa
• GenBank Accession Number: BC018125
• Gene Symbol: UCHL3
• Gene ID (NCBI): 7347
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P15374
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

UCH-L3 belongs to the ubiquitin C-terminal hydrolase family that deubiquitinates ubiquitin-protein conjugates in the ubiquitin-proteasome system. This enzyme indirectly increases the phosphorylation of IGFIR, AKT, FOXO1 and promotes insulin-signaling and insulin-induced adipogenesis. It is required for stress-response retinal, skeletal muscle and germ cell maintenance and may be involved in working memory.