UCHL5 Monoclonal antibody, PBS Only

Name: UCHL5 Monoclonal antibody, PBS Only, 100ug
Brand: Proteintech
SKU: 68535-1-PBS-100UG

UCHL5 Monoclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 68535-1-PBS

Clone No. 2B8G11

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Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

UCH37, 2B8G11, AD-019, CGI 70, CGI70

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

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Filter: AllWBIHC

WB analysis using 68535-1-Ig (same clone as 68535-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 68535-1-Ig (UCHL5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68535-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HCT 116 using 68535-1-Ig (same clone as 68535-1-PBS)
WB result of UCHL5 antibody (68535-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-UCHL5 transfected HCT 116 cells. This data was developed using the same antibody clone with 68535-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse skeletal muscle using 68535-1-Ig (same clone as 68535-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 68535-1-Ig (UCHL5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68535-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human pancreas cancer using 68535-1-Ig (same clone as 68535-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 68535-1-Ig (UCHL5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68535-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human placenta using 68535-1-Ig (same clone as 68535-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 68535-1-Ig (UCHL5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68535-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse pancreas using 68535-1-Ig (same clone as 68535-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 68535-1-Ig (UCHL5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68535-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat kidney using 68535-1-Ig (same clone as 68535-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 68535-1-Ig (UCHL5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68535-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat pancreas using 68535-1-Ig (same clone as 68535-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 68535-1-Ig (UCHL5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68535-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
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Product Information

68535-1-PBS targets UCHL5 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Immunogen	Recombinant protein Predict reactive species
Full Name	ubiquitin carboxyl-terminal hydrolase L5
Calculated Molecular Weight	326 aa, 37 kDa
Observed Molecular Weight	38 kDa
GenBank Accession Number	BC025369
Gene Symbol	UCHL5
Gene ID (NCBI)	51377
RRID	AB_3085243
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q9Y5K5
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

UCHL5 (Ubiquitin carboxyl-terminal hydrolase isozyme L5) is also named as UCH37 and belongs to the peptidase C12 family. It is functionally linked with multiple protein complexes and signal transduction pathways. UCH37's weight average molecular weight increases as a function of its concentration. By size-exclusion chromatography, the apparent molecular weight of UCH37 was 51, 56, 70, and 109 kDa at 0.15, 0.69, 2.4, and 9.5 mg/mL, respectively. Given UCH37's monomeric molecular weight of 37 kDa and its apparent elongated shape, its tendency toward an apparent molecular weight between 40 and 50 kDa at decreasing concentrations is appropriate for UCH37 monomers (PMID:21953935).