UCHL5IP Polyclonal antibody

UCHL5IP Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 17830-1-AP

Synonyms

HAUS7, HSXQ28ORF, UCHL5 interacting protein, UCHL5IP, UIP1, X linked protein STS1769

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Validation Data Gallery

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  WB analysis of MCF-7 using 17830-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 17830-1-AP (UCHL5IP Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary using 17830-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 17830-1-AP (UCHL5IP Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 17830-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 17830-1-AP (UCHL5IP Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of MCF-7 using 17830-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 17830-1-AP (UCHL5IP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: MCF-7 cells
• Positive IHC detected in: human ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

The immunogen of 17830-1-AP is UCHL5IP Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UCHL5IP fusion protein Ag12225
• Full Name: UCHL5 interacting protein
• Calculated Molecular Weight: 368 aa, 41 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC008141
• Gene Symbol: UCHL5IP
• Gene ID (NCBI): 55559
• RRID: AB_2878447
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	J Cell Biol; Reconstitution and mechanistic dissection of the human microtubule branching machinery.; Authors - Yaqian Zhang