Tested Applications
| Positive WB detected in | HEK-293T cells, MCF-7 cells |
| Positive IP detected in | MCF-7 cells |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12271-1-AP targets UCK1 in WB, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2912 Product name: Recombinant human UCK1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-201 aa of BC015547 Sequence: MASAGGEDCESPAPEADRPHQRPFLIGVSGGTASGKSTVCEKIMELLGQNEVEQRQRKVVILSQDRFYKVLTAEQKAKALKGQYNFDHPDAFDNDLMHRTLKNIVEGKTVEVPTYDFVTHSRLPETTVVYPADVVLFEGILVFYSQEIRDMFHLRLFVDTDSDVRLSRRDKEVCRCDHPTRSGQYGCHQPDRAAHPGHSEW Predict reactive species |
| Full Name | uridine-cytidine kinase 1 |
| Calculated Molecular Weight | 277 aa, 31 kDa |
| Observed Molecular Weight | 33 kDa |
| GenBank Accession Number | BC015547 |
| Gene Symbol | UCK1 |
| Gene ID (NCBI) | 83549 |
| RRID | AB_11182173 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9HA47 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Uridine/cytidine kinase-1 is a pyrimidine ribonucleoside kinase that catalyzes the phosphorylation of uridine and cytidine to form uridine monophosphate (UMP) and cytidine monophosphate (CMP). The deduced 277-amino acid protein has a calculated molecular mass of 31 kDa. UCK1 belongs to the uridine kinase family and UCK have important roles for the phosphorylation of nucleoside analogs that may be important in chemotherapy of cancer.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for UCK1 antibody 12271-1-AP | Download protocol |
| IP protocol for UCK1 antibody 12271-1-AP | Download protocol |
| WB protocol for UCK1 antibody 12271-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The UCK1 Polyclonal antibody (Cat# 12271-1-AP) has 10 citations. Journals include Cell Metabolism, Nature Metabolism, Cancer Research, Molecular Biomedicine, Oncogene, Blood Advances, Cell Reports, Molecular Cancer Research, International Journal of Cancer, and PLoS Pathogens. Research fields span pyrimidine metabolism, cancer biology, T-cell immunology, myocardial fibrosis, and anti-SARS-CoV-2 antiviral activity.
| Species | Application | Title |
|---|---|---|
Cell Metab Uridine depletion impairs CD8⁺ T cell antitumor activity through N-glycosylation. | ||
Nat Metab Glucose limitation protects cancer cells from apoptosis induced by pyrimidine restriction and replication inhibition | ||
Cancer Res PAX3-FOXO1 Drives Targetable Cell State-Dependent Metabolic Vulnerabilities in Rhabdomyosarcoma | ||
Mol Biomed Uridine-Cytidine Kinase 2 (UCK2)/Uridine-Cytidine Kinase Like 1 (UCKL1) complex exacerbates the differentiation of myocardial fibroblasts via TRIM21/Smurf2/Smad3 pathway after myocardial infarction. | ||
Oncogene The protection of UCK2 protein stability by GART maintains pyrimidine salvage synthesis for HCC growth under glucose limitation | ||
Blood Adv Reprogramming of pyrimidine nucleotide metabolism supports vigorous cell proliferation of normal and malignant T cells |
Reviews
What customers say
The single review for this antibody (rated 4/5) reports successful use in Western Blot on HEK293T cells at a 1:1000 dilution. The reviewer noted that despite the low expression level of the UCK1 target, the antibody produced a clear, thick band at the expected molecular weight, indicating reliable detection performance under challenging expression conditions.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
H (Verified Customer) (07-09-2020) | This antibody is good considering the low expression of UCK1 protein. I could see a thick band that corresponds with UCK1 MW.
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