Tested Applications
| Positive WB detected in | Jurkat cells |
| Positive IP detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10511-1-AP targets UCK2 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0782 Product name: Recombinant human UCK2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-261 aa of BC002906 Sequence: MAGDSEQTLQNHQQPNGGEPFLIGVSGGTASGKSSVCAKIVQLLGQNEVDYRQKQVVILSQDSFYRVLTSEQKAKALKGQFNFDHPDAFDNELILKTLKEITEGKTVQIPVYDFVSHSRKEETVTVYPADVVLFEGILAFYSQEVRDLFQMKLFVDTDADTRLSRRVLRDISERGRDLEQILSQYITFVKPAFEEFCLPTKKYADVIIPRGADNLVAINLIVQHIQDILNGGPSKRQTNGCLNGYTPSRKRQASESSSRPH Predict reactive species |
| Full Name | uridine-cytidine kinase 2 |
| Calculated Molecular Weight | 29 kDa |
| Observed Molecular Weight | 33-35 kDa |
| GenBank Accession Number | BC002906 |
| Gene Symbol | UCK2 |
| Gene ID (NCBI) | 7371 |
| RRID | AB_2241182 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BZX2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Uridine-cytidine kinase 2 catalyzes the phosphorylation of uridine and cytidine to uridine monophosphate (UMP) and cytidine monophosphate (CMP), respectively. This is the first step in the production of the pyrimidine nucleoside triphosphates required for RNA and DNA synthesis. UCK2 belongs to the uridine kinase family and UCK have important roles for the phosphorylation of nucleoside analogs that may be important in chemotherapy of cancer.
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for UCK2 antibody 10511-1-AP | Download protocol |
| WB protocol for UCK2 antibody 10511-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The UCK2 antibody (Cat# 10511-1-AP) has 26 citations spanning journals including Science, Cancer Cell, Nature Metabolism, Cell Reports, Oncogene, Nucleic Acids Research, Blood Advances, PLoS Pathogens, and Molecular Biomedicine. Research fields encompass pyrimidine metabolism, hepatocellular carcinoma, leukemia, gastric cancer, neuroblastoma, rhabdomyosarcoma, cardiac fibrosis, antiviral bioactivation, T-cell proliferation, and metabolic regulation in cancer. Applications are predominantly WB, with some IHC, IF, and Co-IP.
| Species | Application | Title |
|---|---|---|
Cancer Cell A druggable addiction to de novo pyrimidine biosynthesis in diffuse midline glioma | ||
Nat Metab Salvage of ribose from uridine or RNA supports glycolysis in nutrient-limited conditions
| ||
Nat Metab Glucose limitation protects cancer cells from apoptosis induced by pyrimidine restriction and replication inhibition | ||
Cancer Res PAX3-FOXO1 Drives Targetable Cell State-Dependent Metabolic Vulnerabilities in Rhabdomyosarcoma | ||
Nucleic Acids Res Uridine-cytidine kinase 2 potentiates the mutagenic influence of the antiviral β-d-N4-hydroxycytidine |
Reviews
What customers say
Both reviewers rated the antibody 5 stars for Western Blot. One user found it very cost-effective. The other reported excellent performance using a 1:1000 dilution in 5% BSA with overnight incubation at 4°C, paired with an anti-rabbit IRDye800 secondary antibody and detected on an Odyssey CLx system. The antibody worked well on both HEK293T and HCT116 cell lysates. Overall, customers are satisfied with its sensitivity, ease of use, and value.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Dan (Verified Customer) (05-27-2021) | Very cost effective
|
H (Verified Customer) (04-05-2020) | Primary antibody: 1:1000 dilution in 5% BSA, overnight incubation at 4CSecondary antibody: anti-rabbit IRDye800Detection: Odyssey ClxThis antibody works quite well for HEK293T and HCT116 cell lysates
 |
