UCKL1 Polyclonal antibody

UCKL1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 17005-1-AP

Synonyms

F538, UCK1 LIKE, UCK1L, UCKL1, Uridine cytidine kinase like 1, URKL1

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Validation Data Gallery

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  WB analysis of K-562 using 17005-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 17005-1-AP (UCKL1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 17005-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 17005-1-AP (UCKL1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of U-937 using 17005-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 17005-1-AP (UCKL1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of rat kidney using 17005-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 17005-1-AP (UCKL1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 17005-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17005-1-AP (UCKL1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 17005-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17005-1-AP (UCKL1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 17005-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17005-1-AP (UCKL1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 17005-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17005-1-AP (UCKL1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 17005-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17005-1-AP (UCKL1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 17005-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17005-1-AP (UCKL1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 17005-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17005-1-AP (UCKL1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 17005-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17005-1-AP (UCKL1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 17005-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17005-1-AP (UCKL1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 17005-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17005-1-AP (UCKL1 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: K-562 cells, Raji cells, rat kidney tissue, U-937 cells
• Positive IHC detected in: mouse kidney tissue, human placenta tissue, human testis tissue, human brain tissue, human ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

17005-1-AP targets UCKL1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UCKL1 fusion protein Ag10701
• Full Name: uridine-cytidine kinase 1-like 1
• Calculated Molecular Weight: 419 aa, 46 kDa
• Observed Molecular Weight: 61 kDa, 46 kDa
• GenBank Accession Number: BC033078
• Gene Symbol: UCKL1
• Gene ID (NCBI): 54963
• RRID: AB_2210783
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human,mouse	WB,IHC	EBioMedicine; Non-canonical role of UCKL1 on ferroptosis defence in colorectal cancer; Authors - Weili Wu ; KD Validated