Urocortin Polyclonal antibody

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• Urocortin Polyclonal antibody (19731-1-AP)

Urocortin Polyclonal Antibody for WB, IP, ELISA

Cat No. 19731-1-AP Copied!

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, ELISA

Synonyms

UCN, UI, UROC

• 

  WB analysis using 19731-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19731-1-AP (Urocortin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human testis using 19731-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 19731-1-AP (Urocortin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HEK-293 using 19731-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 19731-1-AP (Urocortin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human kidney using 19731-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 19731-1-AP (Urocortin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of mouse kidney using 19731-1-AP

  IP result of anti-Urocortin (IP:19731-1-AP, 4ug; Detection:19731-1-AP 1:1000) with mouse kidney tissue lysate 2160 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse kidney tissue, HEK-293 cells, human kidney tissue, human testis tissue
• Positive IP detected in: mouse kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19731-1-AP targets Urocortin in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: urocortin
• Calculated Molecular Weight: 13 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: NM_003353
• Gene Symbol: UCN
• Gene ID (NCBI): 7349
• RRID: AB_10640909
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P55089
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Urocortin is a member of the sauvagine/corticotropin-releasing factor/urotensin I family that includes CRF, urotensin I, sauvagine, urocortin II and urocortin III. Urocortin is a potent anorexigenic peptide that induces fed-like motor activity when administered centrally or peripherally in fasted animals. Urocortin is also a potent and long-lasting hypotensive agent and increases coronary blood flow. Urocortin acts in vitro to stimulate the secretion of adrenocorticotropic hormone (ACTH) and binds with high affinity to CRF receptor types 1, 2-alpha, and 2-beta. Urocortin is synthesized as precursor, which is subsequently processed to the mature biologically active peptide (a 40-amino acid Molecule ). The 22 kDa may be a urocortin 1 precursor protein (PMID: 28887029).

Publications

What published studies show

The Urocortin Polyclonal antibody (Cat# 19731-1-AP) has 1 total citation. It was cited in Journal of Cancer Research and Clinical Oncology (2024), in a study investigating how urocortin-1 promotes colorectal cancer cell migration and proliferation while inhibiting apoptosis via the p53 signaling pathway. The antibody was applied in Western blot and immunohistochemistry on human samples. The associated research field is oncology, specifically colorectal cancer biology.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IHC	J Cancer Res Clin Oncol; Urocortin-1 promotes colorectal cancer cell migration and proliferation and inhibits apoptosis via inhibition of the p53 signaling pathway; Authors - Xiaolan Guo