Urocortin Recombinant monoclonal antibody, PBS Only

• 

  WB analysis using 84380-1-RR (same clone as 84380-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84380-1-RR (UCN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84380-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 84380-1-RR (same clone as 84380-1-PBS)

  Rat kidney tissue were subjected to SDS PAGE followed by western blot with 84380-1-RR (UCN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84380-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 84380-1-RR (same clone as 84380-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 84380-1-RR (Urocortin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84380-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 84380-1-RR (same clone as 84380-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 84380-1-RR (Urocortin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84380-1-PBS in a different storage buffer formulation.

Urocortin Uni-rAb^® Recombinant Antibody for WB, IHC, Indirect ELISA

Cat No. 84380-1-PBS

Clone No.241183B12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

UCN, UROC, UI, 241183B12

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Product Information

84380-1-PBS targets Urocortin in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: urocortin
• Calculated Molecular Weight: 13 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: NM_003353
• Gene Symbol: UCN
• Gene ID (NCBI): 7349
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P55089
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Urocortin is a member of the sauvagine/corticotropin-releasing factor/urotensin I family that includes CRF, urotensin I, sauvagine, urocortin II and urocortin III. Urocortin is a potent anorexigenic peptide that induces fed-like motor activity when administered centrally or peripherally in fasted animals. Urocortin is also a potent and long-lasting hypotensive agent and increases coronary blood flow. Urocortin acts in vitro to stimulate the secretion of adrenocorticotropic hormone (ACTH) and binds with high affinity to CRF receptor types 1, 2-alpha, and 2-beta. Urocortin is synthesized as precursor, which is subsequently processed to the mature biologically active peptide (a 40-amino acid Molecule ). The 22 kDa may be a urocortin 1 precursor protein (PMID: 28887029).