Multi-rAb® UCP1 Multi-Recombinant antibody

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• Multi-rAb® UCP1 Multi-Recombinant antibody (RMX00036)

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  WB analysis using RMX00036

  Various lysates were subjected to SDS PAGE followed by western blot with RMX00036 (UCP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brown adipose using RMX00036

  IP result of anti-UCP1 (IP:RMX00036, 4ug; Detection:RMX00036 1:10000) with mouse brown adipose tissue lysate 1280 ug.

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  IHC staining of mouse brown adipose using RMX00036

  Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using RMX00036 (UCP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brown adipose using RMX00036

  Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using RMX00036 (UCP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

UCP1 Multi-Recombinant Antibody for WB, IHC, IP, ELISA

Cat No. RMX00036

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, ELISA

Synonyms

SLC25A7, Thermogenin, UCP, UCP 1

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Tested Applications

• Positive WB detected in: mouse brown adipose tissue
• Positive IP detected in: mouse brown adipose tissue
• Positive IHC detected in: mouse brown adipose tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

RMX00036 targets UCP1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Multi-Recombinant
• Type: Antibody
• Immunogen:

  Mixture

• Full Name: uncoupling protein 1 (mitochondrial, proton carrier)
• Calculated Molecular Weight: 307 aa, 33 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC098168
• Gene Symbol: UCP1
• Gene ID (NCBI): 7350
• RRID: AB_3750090
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: N/A
• UNIPROT ID: P25874
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.