UCP3 Polyclonal antibody

UCP3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10750-1-AP

Synonyms

SLC25A9, UCP 3, UCP3



Tested Applications

Positive WB detected inmouse skeletal muscle tissue, SGC-7901 cells, rat skeletal muscle tissue
Positive IHC detected inhuman skeletal muscle tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10750-1-AP targets UCP3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UCP3 fusion protein Ag1134
Full Name uncoupling protein 3 (mitochondrial, proton carrier)
Calculated Molecular Weight 34 kDa
Observed Molecular Weight 30-34 kDa
GenBank Accession NumberBC008392
Gene Symbol UCP3
Gene ID (NCBI) 7352
RRIDAB_2272729
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for UCP3 antibody 10750-1-APDownload protocol
IHC protocol for UCP3 antibody 10750-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Cell

DNA Damage Promotes TMPRSS2-ERG Oncoprotein Destruction and Prostate Cancer Suppression via Signaling Converged by GSK3β and WEE1.

Authors - Zhe Hong
mouseWB

Circ Res

Uncoupling protein 2 deficiency mimics the effects of hypoxia and endoplasmic reticulum stress on mitochondria and triggers pseudohypoxic pulmonary vascular remodeling and pulmonary hypertension.

Authors - Dromparis Peter P
mouseWB

EBioMedicine

Maternal exercise intergenerationally drives muscle-based thermogenesis via activation of apelin-AMPK signaling.

Authors - Jun Seok Son
ratWB,IHC

Cell Physiol Biochem

PMID: 29669326

Authors - Yintao Chen
mouseWB

J Mol Cell Cardiol

Impaired SIRT3 activity mediates cardiac dysfunction in endotoxemia by calpain-dependent disruption of ATP synthesis.

Authors - Christoph Koentges
mouseWB

Sci Rep

Reduction in the levels of CoQ biosynthetic proteins is related to an increase in lifespan without evidence of hepatic mitohormesis.

Authors - María Rodríguez-Hidalgo