UFC1 Polyclonal antibody

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• KD/KO Validated

UFC1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 15783-1-AP

Synonyms

HSPC155, UFC1, Ufm1 conjugating enzyme 1

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Validation Data Gallery

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  WB analysis using 15783-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15783-1-AP (UFC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 15783-1-AP

  WB result of UFC1 antibody (15783-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-UFC1 transfected A549 cells.

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  IHC staining of human liver using 15783-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15783-1-AP (UFC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 15783-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15783-1-AP (UFC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A549 cells, HepG2 cells, MCF-7 cells, mouse kidney tissue, rat kidney tissue
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

15783-1-AP targets UFC1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UFC1 fusion protein Ag8480
• Full Name: ubiquitin-fold modifier conjugating enzyme 1
• Calculated Molecular Weight: 167 aa, 19 kDa
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: BC005187
• Gene Symbol: UFC1
• Gene ID (NCBI): 51506
• RRID: AB_2213938
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The function of UFC1 (ubiquitin fold modifier conjugating enzyme 1) has not been widely studied, and is yet to be fully elucidated. The MW of this protein is 21 kDa, and this antibody specially recognises the 21 kDa protein. Catalog#15783-1-AP specially recognises the 21 kDa protein.

Publications

Species	Application	Title
		Mol Cell; An Epstein-Barr virus protein interaction map reveals NLRP3 inflammasome evasion via MAVS UFMylation; Authors - Stephanie Pei Tung Yiu
human	WB	J Biol Chem; Mechanistic Study of Uba5 and the Ufm1 Conjugation Pathway.; Authors - James M Gavin
mouse	WB	BMC Cardiovasc Disord; Screening UFMylation-associated genes in heart tissues of Ufm1-transgenic mice; Authors - Hu Jiajia