UFSP1 Polyclonal antibody

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  WB analysis using 14655-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14655-1-AP (UFSP1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

UFSP1 Polyclonal Antibody for WB, ELISA

Cat No. 14655-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

UFSP, Inactive Ufm1-specific protease 1

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Tested Applications

• Positive WB detected in: HEK-293T cells, LNCaP cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14655-1-AP targets UFSP1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6256

  Product name: Recombinant human UFSP1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-142 aa of BC067904

  Sequence: MGDKPPGFRGSRDWIGCVEASLCLAHFGGPQGRLCHVPRGVGLHGEVERLYSHFAGGGGPVMVGGDADARSKALLGVCVGSGTEAYVLVLDPHYWGTPKSPSELQAAGWVGWQEVSAAFDPNSFYNLCLTSLSSQQQQRTLD

• Full Name: UFM1-specific peptidase 1 (non-functional)
• Calculated Molecular Weight: 15 kDa
• Observed Molecular Weight: 23 kDa
• GenBank Accession Number: BC067904
• Gene Symbol: UFSP1
• Gene ID (NCBI): 402682
• RRID: AB_3669192
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6NVU6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UFSP1 is the Thiol-dependent isopeptidase that specifically mediate the processing of UFM1 precursors as well as the deconjugation of UFM1 from target proteins (PMID: 35525273; 35926457).