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  • KD/KO Validated

UGCG Polyclonal antibody

UGCG Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 12869-1-AP

Synonyms

Ceramide glucosyltransferase, GCS, GLCT 1, Glucosylceramide synthase, UGCG



Tested Applications

Positive WB detected inA375 cells, C6 cells, mouse brain tissue, U-251 cells
Positive IP detected inA375 cells
Positive IHC detected inhuman lung cancer tissue, human breast cancer tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

12869-1-AP targets UGCG in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UGCG fusion protein Ag3530
Full Name UDP-glucose ceramide glucosyltransferase
Calculated molecular weight 394 aa, 45 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC038711
Gene symbol UGCG
Gene ID (NCBI) 7357
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

UGCG, also named as GCS and GLCT-1, belongs to the glycosyltransferase 2 family. It catalyzes the first glycosylation step in glycosphingolipid biosynthesis, the transfer of glucose to ceramide. UGCG may also serve as a "flippase". It is up-regulated at the transcriptional level during keratinocyte differentiation. UGCG is a membrane protein. The calculated MW is 45 kDa. In SDS-PAGE, it is about 50-55 kDa.

Protocols

Product Specific Protocols
WB protocol for UGCG antibody 12869-1-APDownload protocol
IHC protocol for UGCG antibody 12869-1-APDownload protocol
IP protocol for UGCG antibody 12869-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Aging (Albany NY)

Identification of long-term survival-associated gene in breast cancer.

Authors - Shipeng Ning
humanWB

Front Pharmacol

A Novel Drug Resistance Mechanism: Genetic Loss of Xeroderma Pigmentosum Complementation Group C (XPC) Enhances Glycolysis-Mediated Drug Resistance in DLD-1 Colon Cancer Cells.

Authors - Yu Han
human, mouseWB,IHC

Hum Mol Genet

Amyotrophic lateral sclerosis and denervation alter sphingolipids and up-regulate glucosylceramide synthase.

Authors - Alexandre Henriques
human,mouseWB

Cancer Res

Aneuploid cell survival relies upon sphingolipid homeostasis.

Authors - Yun-Chi Tang
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Will (Verified Customer) (01-31-2019)

good antibody, would order again

  • Applications: Western Blot,