Validation Data Gallery
|Positive WB detected in||A375 cells, C6 cells, mouse brain tissue, U-251 cells|
|Positive IP detected in||A375 cells|
|Positive IHC detected in||human lung cancer tissue, human breast cancer tissue, human lymphoma tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
12869-1-AP targets UGCG in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||UGCG fusion protein Ag3530|
|Full Name||UDP-glucose ceramide glucosyltransferase|
|Calculated molecular weight||394 aa, 45 kDa|
|Observed molecular weight||50-55 kDa|
|GenBank accession number||BC038711|
|Gene ID (NCBI)||7357|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
UGCG, also named as GCS and GLCT-1, belongs to the glycosyltransferase 2 family. It catalyzes the first glycosylation step in glycosphingolipid biosynthesis, the transfer of glucose to ceramide. UGCG may also serve as a "flippase". It is up-regulated at the transcriptional level during keratinocyte differentiation. UGCG is a membrane protein. The calculated MW is 45 kDa. In SDS-PAGE, it is about 50-55 kDa.
Glycolysis regulates KRAS plasma membrane localization and function through defined glycosphingolipids
Aneuploid cell survival relies upon sphingolipid homeostasis.
Hum Mol Genet
Amyotrophic lateral sclerosis and denervation alter sphingolipids and up-regulate glucosylceramide synthase.
Aging (Albany NY)
Identification of long-term survival-associated gene in breast cancer.
Liver sphingomyelin synthase 1 deficiency causes steatosis, steatohepatitis, fibrosis, and tumorigenesis: An effect of glucosylceramide accumulation.
A Novel Drug Resistance Mechanism: Genetic Loss of Xeroderma Pigmentosum Complementation Group C (XPC) Enhances Glycolysis-Mediated Drug Resistance in DLD-1 Colon Cancer Cells.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Will (Verified Customer) (01-31-2019)
good antibody, would order again