UGCGL1 Polyclonal antibody

UGCGL1 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 14170-1-AP

Synonyms

GT, HUGT1, UGCGL1, UGGT, UGGT1, UGT1, UGTR



Tested Applications

Positive WB detected inHeLa cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman pancreas cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

14170-1-AP targets UGCGL1 in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UGCGL1 fusion protein Ag5369
Full Name UDP-glucose ceramide glucosyltransferase-like 1
Calculated molecular weight 1531 aa, 175 kDa
Observed molecular weight 170 kDa
GenBank accession numberBC041098
Gene symbol UGCGL1
Gene ID (NCBI) 56886
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for UGCGL1 antibody 14170-1-APDownload protocol
IHC protocol for UGCGL1 antibody 14170-1-APDownload protocol
IP protocol for UGCGL1 antibody 14170-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Biochem Biophys Res Commun

Arginine induces IGF-1 secretion from the endoplasmic reticulum.

Authors - Yoji Tsugawa
mouseWB

Biochem Biophys Res Commun

UGGT1 retains proinsulin in the endoplasmic reticulum in an arginine dependent manner.

Authors - Jaeyong Cho
humanWB

Mol Cell Proteomics

Thyroglobulin Interactome Profiling Defines Altered Proteostasis Topology Associated With Thyroid Dyshormonogenesis.

Authors - Madison T Wright