UGT1A10 Polyclonal antibody

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  WB analysis using 14457-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14457-1-AP (UGT1A10 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

UGT1A10 Polyclonal Antibody for WB, ELISA

Cat No. 14457-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

UGT1*10, UDPGT, UDP-glucuronosyltransferase 1-J, UDP-glucuronosyltransferase 1A10, UDP-glucuronosyltransferase 1-10

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Tested Applications

• Positive WB detected in: HEK-293T cells, HeLa cells, L02 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14457-1-AP targets UGT1A10 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6052

  Product name: Recombinant human UGT1A10 protein

  Source: e coli.-derived, PKG

  Tag: GST

  Domain: 1-350 aa of BC053576

  Sequence: MARAGWTSPVPLCVCLLLTCGFAEAGKLLVVPMDGSHWFTMQSVVEKLILRGHEVVVVMPEVSWQLERSLNCTVKTYSTSYTLEDQNREFMVFAHAQWKAQAQSIFSLLMSSSSGFLDLFFSHCRSLFNDRKLVEYLKESSFDAVFLDPFDTCGLIVAKYFSLPSVVFTRGIFCHHLEEGAQCPAPLSYVPNDLLGFSDAMTFKERVWNHIVHLEDHLFCQYLFRNALEIASEILQTPVTAYDLYSHTSIWLLRTDFVLDYPKPVMPNMIFIGGINCHQGKPLPMEFEAYINASGEHGIVVFSLGSMVSEIPEKKAMAIADALGKIPQTVLWRYTGTRPSNLANNTILVK

• Full Name: UDP glucuronosyltransferase 1 family, polypeptide A10
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC053576
• Gene Symbol: UGT1A10
• Gene ID (NCBI): 54575
• RRID: AB_3669187
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9HAW8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UGT1A10 belongs to the superfamily of UDP-glucuronosyltransferases (UGTs) catalyzing the glucuronidation reaction, which is important in the detoxification and enhanced elimination of many endogenous and exogenous substrates. Recently, UGT1A10 was the only enzyme in all 19 known human UGTs to catalyze DA glucuronidation. The UGT1As, among three subfamilies (1A, 2A, and 2B) found in human, share a common C-terminal domain of 245 residues. The N-terminal domains that determine their substrate-binding specificity are variable.