Tested Applications
| Positive WB detected in | A431 cells, HeLa cells, HepG2 cells, L02 cells, mouse liver tissue, rat liver tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16663-1-AP targets UGT1A9 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10139 Product name: Recombinant human UGT1A9 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 33-377 aa of BC058844 Sequence: MDGSHWFTMRSVVEKLILRGHEVVVVMPEVSWQLGRSLNCTVKTYSTSYTLEDLDREFKAFAHAQWKAQVRSIYSLLMGSYNDIFDLFFSNCRSLFKDKKLVEYLKESSFDAVFLDPFDNCGLIVAKYFSLPSVVFARGILCHYLEEGAQCPAPLSYVPRILLGFSDAMTFKERVRNHIMHLEEHLLCHRFFKNALEIASEILQTPVTEYDLYSHTSIWLLRTDFVLDYPKPVMPNMIFIGGINCHQGKPLPMEFEAYINASGEHGIVVFSLGSMVSEIPEKKAMAIADALGKIPQTVLWRYTGTRPSNLANNTILVKWLPQNDLLGHPMTRAFITHAGSHGVYE Predict reactive species |
| Full Name | UDP glucuronosyltransferase 1 family, polypeptide A9 |
| Calculated Molecular Weight | 530 aa, 60 kDa |
| Observed Molecular Weight | 60-70 kDa |
| GenBank Accession Number | BC058844 |
| Gene Symbol | UGT1A9 |
| Gene ID (NCBI) | 54600 |
| RRID | AB_3669254 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O60656 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
UDP-glucuronosyltransferase 1A9 (UGT1A9) is an enzyme that plays a crucial role in the glucuronidation pathway. It transforms small lipophilic molecules, such as steroids, bilirubin, hormones, and drugs, into water-soluble, excretable metabolites. UGT1A9 is part of a complex locus that encodes several UDP-glucuronosyltransferases. The enzyme is active on phenols and is involved in the metabolism of various drugs and endogenous compounds (PMID: 15284532; 12181437; 18004212).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for UGT1A9 antibody 16663-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The UGT1A9 Polyclonal antibody (Cat# 16663-1-AP) has 2 total citations. These citations appear in Cancer Cell International and Biochemical Pharmacology. The associated research fields include oncology (glioblastoma cell proliferation and apoptosis) and pharmacology (hepatic drug metabolism and sleep deprivation effects on UGT1A9-mediated propofol metabolism). Both studies utilized the antibody for Western blot applications in human and mouse samples, respectively.
| Species | Application | Title |
|---|---|---|
Cancer Cell Int 4,5-Dimethoxycanthin-6-one is a novel LSD1 inhibitor that inhibits proliferation of glioblastoma cells and induces apoptosis and pyroptosis. | ||
Biochem Pharmacol Sleep deprivation alters hepatic UGT1A9 and propofol metabolism in mice |
