UGT2B7 Polyclonal antibody

UGT2B7 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

15

Cat no : 16661-1-AP

Synonyms

UDPGT 2B9, UDPGT 2B7, UDP-glucuronosyltransferase 2B9, UDP-glucuronosyltransferase 2B7, EC:2.4.1.17

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Validation Data Gallery

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  WB analysis using 16661-1-AP

  Rat liver lysates were subjected to SDS PAGE followed by western blot with 16661-1-AP (UGT2B7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 16661-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16661-1-AP (UGT2B7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of rat liver using 16661-1-AP

  IP result of anti-UGT2B7 (IP:16661-1-AP, 4ug; Detection:16661-1-AP 1:3000) with rat liver tissue lysate 4000 ug.

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  IHC staining of human liver using 16661-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16661-1-AP (UGT2B7 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver using 16661-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16661-1-AP (UGT2B7 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 16661-1-AP

  Immunofluorescent analysis of HepG2 cells, using UGT2B7 antibody 16661-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: rat liver tissue, HEK-293 cells
• Positive IP detected in: rat liver tissue
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 14 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Product Information

16661-1-AP targets UGT2B7 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UGT2B7 fusion protein Ag10121
• Full Name: UDP glucuronosyltransferase 2 family, polypeptide B7
• Calculated Molecular Weight: 529 aa, 61 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC030974
• Gene Symbol: UGT2B7
• Gene ID (NCBI): 7364
• RRID: AB_2214249
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Cell Rep; Divergent Expression and Metabolic Functions of Human Glucuronosyltransferases through Alternative Splicing.; Authors - Michèle Rouleau
human	WB	Br J Pharmacol; UDP-Glucuronosyltransferase (UGT)-Mediated Attenuations of Cytochrome P450 3A4 Activity: UGT Isoform-Dependent Mechanism of Suppression.; Authors - Yuu Miyauchi
	WB	Sci Rep; Inter-isoform Hetero-dimerization of Human UDP-Glucuronosyltransferases (UGTs) 1A1, 1A9, and 2B7 and Impacts on Glucuronidation Activity.; Authors - Ling-Min Yuan
human	WB	Sci Rep; Quantitative proteomics by SWATH-MS reveals sophisticated metabolic reprogramming in hepatocellular carcinoma tissues.; Authors - Yanyan Gao
	WB	Biochem Pharmacol; PMID: 25770680; Authors - Lingmin Yuan
	WB	Front Pharmacol; Use of a Baculovirus-Mammalian Cell Expression-System for Expression of Drug-Metabolizing Enzymes: Optimization of Infection With a Focus on Cytochrome P450 3A4.; Authors - Yuu Miyauchi