UGT8 Polyclonal antibody

• Featured Product
• KD/KO Validated

UGT8 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA, IF

Conjugate

Unconjugated

Publications

9

Cat no : 17982-1-AP

Synonyms

UGT 8, UDP-galactose-ceramide galactosyltransferase, EC:2.4.1.47, CGT, Ceramide UDP-galactosyltransferase

---

Validation Data Gallery

• 

  WB analysis of rat brain using 17982-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 17982-1-AP (UGT8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 17982-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 17982-1-AP (UGT8 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of rat brain using 17982-1-AP

  IP result of anti-UGT8 (IP:17982-1-AP, 4ug; Detection:17982-1-AP 1:1000) with rat brain tissue lysate 4000ug.

• 

  IHC staining of human brain using 17982-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17982-1-AP (UGT8 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 17982-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17982-1-AP (UGT8 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human breast cancer using 17982-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17982-1-AP (UGT8 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 17982-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17982-1-AP (UGT8 Antibody) at dilution of 1:50 (under 40x lens).

---

Tested Applications

• Positive WB detected in: rat brain tissue
• Positive IP detected in: rat brain tissue
• Positive IHC detected in: human brain tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 5 publications below
• IF: See 1 publications below

Product Information

17982-1-AP targets UGT8 in WB, IHC, IP, ELISA, IF applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UGT8 fusion protein Ag12393
• Full Name: UDP glycosyltransferase 8
• Calculated Molecular Weight: 541 aa, 61 kDa
• Observed Molecular Weight: 61 kDa
• GenBank Accession Number: BC075069
• Gene Symbol: UGT8
• Gene ID (NCBI): 7368
• RRID: AB_2214254
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UGT8, also known as galacotosylceramide synthase or CGT (ceramide galactosyltransferase), is a key enzyme for galacotosylceramide (GalCer) biosynthesis. It is an ER transmembrane protein and has limited tissue distribution, with predominance in Schwann cells, oligodendrocytes, kidneys, testes, and intestine. UGT8 is highly enriched in myelin in the central nervous system. Its expression levels are strongly associated with histological typing in human oligodendrogliomas and astrocytomas; can be used as molecular marker to distinguish these tumors. Higher UGT8 levels had also been linked to metastatic properties of cancer cells. This antibody specifically recognizes the endogenous UGT8.

Publications

Species	Application	Title
human	WB,IF	J Exp Med; Inhibition of UGT8 suppresses basal-like breast cancer progression by attenuating sulfatide-αVβ5 axis.; Authors - Qianhua Cao ; KD Validated
mouse,human	WB,IHC	Mol Cell Proteomics; Metabolic Enzyme Alterations and Astrocyte Dysfunction in a Murine Model of Alexander Disease With Severe Reactive Gliosis.; Authors - Michael R Heaven
human	WB	Mol Pharmacol; Circular RNAs of UDP-Glycosyltransferase (UGT) Genes Expand the Complexity and Diversity of the UGT Transcriptome.; Authors - Dong Gui Hu
human	IHC	Biosci Rep; Uronic acid metabolic process-related gene expression-based signature predicts overall survival of glioma.; Authors - Yuemei Feng
rat, human	WB,IHC	Cancer Chemother Pharmacol; Evaluation of resveratrol sensitivities and metabolic patterns in human and rat glioblastoma cells.; Authors - Sun Zheng Z
rat	WB, IHC	Neurotherapeutics; Diffusion Efficiency and Bioavailability of Resveratrol Administered to Rat Brain by Different Routes: Therapeutic Implications.; Authors - Xiao-Hong Shu