UHMK1 Polyclonal antibody

• Featured Product
• KD/KO Validated

UHMK1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 11624-1-AP

Synonyms

KIS, Kist, U2AF homology motif kinase 1, UHMK1

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Validation Data Gallery

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  WB analysis of mouse thymus using 11624-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11624-1-AP (UHMK1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 11624-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11624-1-AP (UHMK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 11624-1-AP

  IP result of anti-UHMK1 (IP:11624-1-AP, 4ug; Detection:11624-1-AP 1:500) with mouse brain tissue lysate 2640ug.

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  IHC staining of human stomach using 11624-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 11624-1-AP (UHMK1 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human stomach using 11624-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 11624-1-AP (UHMK1 antibody) at dilution of 1:200 (under 10x lens).

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Tested Applications

• Positive WB detected in: mouse thymus tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 4 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Product Information

11624-1-AP targets UHMK1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UHMK1 fusion protein Ag2204
• Full Name: U2AF homology motif (UHM) kinase 1
• Calculated Molecular Weight: 419 aa, 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC026046
• Gene Symbol: UHMK1
• Gene ID (NCBI): 127933
• RRID: AB_2214267
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UHMK1, also named as KIS, or KIST, is a 419 amino acid protein, which contains one RRM (RNA recognition motif) domain, one protein kinase domain, and belongs to the protein kinase superfamily. UHMK1 is widely expressed, with highest levels in skeletal muscle, kidney, placenta and peripheral blood leukocytes. UHMK1 localizes in the nucleus and upon serum stimulation, phosphorylating CDKN1B/p27Kip1, thus controlling CDKN1B subcellular location and cell cycle progression in G1 phase. UHMK1 may be involved in trafficking and/or processing of RNA and may have a role in the developing nervous system and the adult brain.

Publications

Species	Application	Title
rat	IHC	Brain Res; Cord blood administration induces oligodendrocyte survival through alterations in gene expression.; Authors - Rowe D D DD
human	WB,IHC,IF	Cell Death Dis; UHMK1 aids colorectal cancer cell proliferation and chemoresistance through augmenting IL-6/STAT3 signaling.; Authors - Xuedi Gao ; KD Validated
human	WB	Thorac Cancer; UHMK1 promotes lung adenocarcinoma oncogenesis by regulating the PI3K/AKT/mTOR signaling pathway; Authors - Yongmeng Li ; KD Validated
human	WB,IHC	J Cancer Res Clin Oncol; KIS, a target of SOX4, regulates the ID1-mediated enhancement of β-catenin to facilitate lung adenocarcinoma cell proliferation and metastasis; Authors - Jing-Xia Chang ; KD Validated
human	WB,IHC	Oral Surg Oral Med Oral Pathol Oral Radiol; Expression and clinical significance of U2AF homology motif kinase 1 in oral squamous cell carcinoma; Authors - Xuan Zhang