UHRF1BP1L Polyclonal antibody

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  WB analysis using 25121-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25121-1-AP (UHRF1BP1L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 25121-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25121-1-AP (UHRF1BP1L Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 25121-1-AP

  IP result of anti-UHRF1BP1L (IP:25121-1-AP, 5ug; Detection:25121-1-AP 1:1000) with mouse brain tissue lysate 5000ug.

UHRF1BP1L Polyclonal Antibody for WB, IP, ELISA

Cat No. 25121-1-AP

Publications(1)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Synonyms

BLTP3B, KIAA0701, SHIP164

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

25121-1-AP targets UHRF1BP1L in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag19141

  Product name: Recombinant human UHRF1BP1L protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1089-1440 aa of BC127017

  Sequence: HAHEQDTKMHEIYKGNITPQLNKNTLKTSAATDVWAVYFSQFWIDYEGMKSGKGRPISFVDSFPLSIWICQPTRYAESQKEPQTCNQVSLNTSQSESSDLAGRLKRKKLLKEYYSTESEPLTNGGQKPSSSDTFFRFSPSSSEADIHLLVHVHKHVSMQINHYQYLLLLFLHESLILLSENLRKDVEAVTGSPASQTSICIGILLRSAELALLLHPVDQANTLKSPVSESVSPVVPDYLPTENGDFLSSKRKQISRDINRIRSVTVNHMSDNRSMSVDLSHIPLKDPLLFKSASDTNLQKGISFMDYLSDKHLGKISEDESSGLVYKSGSGEIGSETSDKKDSFIQIQVVS

• Full Name: UHRF1 binding protein 1-like
• Calculated Molecular Weight: 1464 aa, 164 kDa
• Observed Molecular Weight: 160-165 kDa
• GenBank Accession Number: BC127017
• Gene Symbol: UHRF1BP1L
• Gene ID (NCBI): 23074
• RRID: AB_2879909
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: A0JNW5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	iScience; Combining light-induced aggregation and biotin proximity labeling implicates endolysosomal proteins in early α-synuclein oligomerization.; Authors - Maxime Teixeira

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Daniel (Verified Customer) (10-26-2022)

This antibody works well and produced little background. Works best in PVDF membranes.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HeLa, Mt4 cells

UHRF1BP1L Antibody Western Blot validation (1:1000 dilution) in HeLa, Mt4 cells (Cat no:25121-1-AP)