UHRF2 Polyclonal antibody, PBS Only

• Home
• Products
• Primary Antibodies
• UHRF2 Polyclonal antibody, PBS Only (25710-1-PBS)

• 

  WB analysis using 25710-1-AP (same clone as 25710-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 25710-1-AP (UHRF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25710-1-PBS in a different storage buffer formulation.

• 

  IP experiment of Jurkat using 25710-1-AP (same clone as 25710-1-PBS)

  IP result of anti-UHRF2 (IP:25710-1-AP, 4ug; Detection:25710-1-AP 1:300) with Jurkat cells lysate 4000ug. This data was developed using the same antibody clone with 25710-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 25710-1-AP (same clone as 25710-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using UHRF2 antibody (25710-1-AP) at dilution of 1:800 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25710-1-PBS in a different storage buffer formulation.

UHRF2 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Cat No. 25710-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, Indirect ELISA

Synonyms

---

Product Information

25710-1-PBS targets UHRF2 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag22519

  Product name: Recombinant human UHRF2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 77-117 aa of BC028397

  Sequence: PDHLPGTSTQIEAKPCSNSPPKVKKAPRVGPSNQPSTSARA

• Full Name: ubiquitin-like with PHD and ring finger domains 2
• Calculated Molecular Weight: 90 kDa
• Observed Molecular Weight: 90 kDa
• GenBank Accession Number: BC028397
• Gene Symbol: UHRF2
• Gene ID (NCBI): 115426
• RRID: AB_2935463
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96PU4
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Ubiquitin-Like with PHD and ring finger domains 2 (UHRF2), a member that belongs to the family of UHRF, contains five recognizable functional domains, namely the ubiquitin-like domain (UBL) domain, tandem-Tudor domain (TTD), plant homeodomain (PHD), SET and RING associated (SRA) domain, and really interesting new gene (RING) finger domain. Due to the complex structure, UHRF2 possesses multiple functions in diverse cellular processes. As a ubiquitin E3 ligase, UHRF2 could ubiquitinate PCNP, a nuclear protein that contains two remarkable PEST sequences which are rich in proline (P), glutamic acid (E), serine (S), and threonine (T). It has been also reported that UHRF2 could serve as a vital cell cycle regulator by interacting with multiple cyclins, CDKs, p53, pRB and PCNA. UHRF2 has been revealed to possess epigenetic regulation function and is capable of maintaining 5mC levels in certain genomic loci in brain and stabilizes TIP60 to regulate H3K9ac and H3K14ac through RING finger domain. Moreover, UHRF2 could promote DNA damage repair by reducing the level of p21 mediated by RING finger domain. Recently, emerging evidence indicated that UHRF2 was involved in the tumorigenesis and progression of several human cancers, such as esophageal squamous cell carcinoma, lung cancer and colorectal cancer. UHRF2 has 2 isoforms with the molecular mass of 56 and 90 kDa. (PMID: 34400880)