ULBP2 Polyclonal antibody

ULBP2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 13133-1-AP

Synonyms

ALCAN alpha, N2DL 2, N2DL2, NKG2D ligand 2, NKG2DL2, RAET1H, UL16 binding protein 2, ULBP2

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Validation Data Gallery

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  WB analysis of Jurkat using 13133-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13133-1-AP (ULBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 13133-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13133-1-AP (ULBP2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 13133-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13133-1-AP (ULBP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 13133-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13133-1-AP (ULBP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 13133-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13133-1-AP (ULBP2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Jurkat cells
• Positive IHC detected in: human liver cancer tissue, human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

13133-1-AP targets ULBP2 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ULBP2 fusion protein Ag3798
• Full Name: UL16 binding protein 2
• Calculated Molecular Weight: 246 aa, 27 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC034689
• Gene Symbol: ULBP2
• Gene ID (NCBI): 80328
• RRID: AB_2214691
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NKG2D is an activating cell surface receptor that is predominantly expressed on cytotoxic immune cells. NKG2D recognizes a wide range of ligands. In humans, the NKG2D ligand (NKG2DL) includes MICA, MICB, and six members of the ULBP family (ULBP1-6) (PMID: 29568297; 30813924). ULBPs are MHC class I-related molecules (PMID: 11239445). ULBP2 contains two Ig-like domains, the alpha-1 and alpha-2 domains characteristic of the MHC class I family, but lacks the alpha-3 domain. It can be expressed as either a transmembrane or GPI-linked protein, or released from the cell surface (PMID: 21224393; 24614922). ULBP2 binds and activates the NKG2D, mediating the recruitment and activation of NK cells.

Publications

Species	Application	Title
human	WB	Cancer Lett; Pleckstrin-2 promotes tumour immune escape from NK cells by activating the MT1-MMP-MICA signalling axis in gastric cancer; Authors - Deli Mao
human	WB	Int J Biol Sci; CD44 3'-Untranslated Region Functions as a Competing Endogenous RNA to Enhance NK Sensitivity of Liver Cancer Stem Cell by Regulating ULBP2 Expression.; Authors - Jun Weng
human	WB	Int Immunopharmacol; Venetoclax enhances NK cell killing sensitivity of AML cells through the NKG2D/NKG2DL activation pathway.; Authors - Hui-Yang Wu