Tested Applications
| Positive WB detected in | HeLa cells |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
29005-1-AP targets ULK1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human, mouse, rat, goat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | unc-51-like kinase 1 (C. elegans) |
| Observed Molecular Weight | 130 kDa |
| GenBank Accession Number | NM_003565 |
| Gene Symbol | ULK1 |
| Gene ID (NCBI) | 8408 |
| RRID | AB_2918228 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75385 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ULK1, also named as KIAA0722, belongs to the protein kinase superfamily, Ser/Thr protein kinase family and APG1/unc-51/ULK1 subfamily. It is involved in autophagy. ULK1 is required for autophagosome formation. It forms a stable complex with Atg13 and focal adhesion kinase (FAK) family interacting protein of 200 kDa (FIP 200)(PMID:21460634). ULK1 phosphorylates ATG13/KIAA0652. It is involved in axon growth. ULK1 plays an essential role in neurite extension of cerebellar granule cells. ULK1 represents a potential novel prognostic biomarker for HCC patients and may play an important role during the progression of HCC(PMID:23573318).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ULK1 antibody 29005-1-AP | Download protocol |
| WB protocol for ULK1 antibody 29005-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ULK1 Polyclonal antibody (Cat# 29005-1-AP) has 13 citations published in journals including Autophagy, Advanced Science, Cell Death & Differentiation, Science China Life Sciences, Free Radical Biology and Medicine, Aging Cell, Cancer Cell International, Ecotoxicology and Environmental Safety, Scientific Reports, Medical Oncology, Journal of Cancer Research and Clinical Oncology, Reproductive Sciences, and CytoJournal. Research fields span autophagy regulation, cancer (gastric, bladder, pancreatic, esophageal), cardiac disease, ferroptosis, and cellular senescence.
| Species | Application | Title |
|---|---|---|
Autophagy Regulation of N-Degron Recognin-Mediated Autophagy by the sars-cov-2 plpro ubiquitin deconjugase | ||
Adv Sci (Weinh) Aloe Emodin Alleviates Radiation-Induced Heart Disease via Blocking P4HB Lactylation and Mitigating Kynurenine Metabolic Disruption | ||
Cell Death Differ TTK promotes mitophagy by regulating ULK1 phosphorylation and pre-mRNA splicing to inhibit mitochondrial apoptosis in bladder cancer | ||
Free Radic Biol Med LncRNA BASP1-AS1 drives PCBP2 K115 lactylation to suppress ferroptosis and confer oxaliplatin resistance in gastric cancer | ||
Aging Cell DLK/JNK3 Upregulation Aggravates Hair Cell Senescence in Mice Cochleae via Excessive Autophagy |
