UMP/CMP kinase Recombinant monoclonal antibody, PBS Only

• 

  WB analysis using 86475-3-RR (same clone as 86475-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86475-3-RR (CMPK1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86475-3-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 86475-3-RR

  Biolayer interferometry (BLl) kinetic assays of 86475-3-RR against Human UMP/CMP kinase were performed. The affinity constant is below 1 pM.

UMP/CMP kinase Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 86475-3-PBS

Clone No.251233A1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

CMPK1, CK, CMK, CMPK, Cytidine monophosphate kinase

---

Product Information

86475-3-PBS targets UMP/CMP kinase in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag1920

  Product name: Recombinant human CMPK1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-228 aa of BC014961

  Sequence: MLSRCRSRLLHVLGLSFLLQTRRPILLCSPRLMKPLVVFVLGGPGAGKGTQCARIVEKYGYTHLSAGELLRDERKNPDSQYGELIEKYIKEGKIVPVEITISLLKREMDQTMAANAQKNKFLIDGFPRNQDNLQGWNKTMDGKADVSFVLFFDCNNEICIERCLERGKSSGRSDDNRESLEKRIQTYLQSTKPIIDLYEEMGKVKKIDASKSVDEVFDEVVQIFDKEG

• Full Name: cytidine monophosphate (UMP-CMP) kinase 1, cytosolic
• Calculated Molecular Weight: 228 aa, 26 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC014961
• Gene Symbol: CMPK1
• Gene ID (NCBI): 51727
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P30085
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Pyrimidine nucleoside monophosphate kinase [UMP/CMP kinase (UMP/CMPK)] (CMPK1) is also named as CMK, CMPK, UCK, UMK, UMPK and belongs to the adenylate kinase family. It plays a crucial role in the formation of UDP, CDP, and dCDP, which are required for cellular nucleic acid synthesis(PMID:11912132). CMPK1 can use a broad spectrum of phosphate donors but the best phosphate donors are ATP and dATP. CMPK1 has been reported as a prognostic marker for non-small cell lung cancer, pancreatic cancer and breast cancer (PMID: 21642870, 22838950, 27558661). The longest deduced protein contains 228 amino acids and has a calculated molecular mass of 26 kD. However, translation likely begins at the second initiation methionine, resulting in a protein of 196 amino acids(PMID:10462544). The shorter form is the endogenously translated protein(PMID:11912132). CMPK1 has 3 isoforms with the molecular mass of 22, 16 and 26 kDa.