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  • KD/KO Validated

UMPS Polyclonal antibody

UMPS Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 14830-1-AP

Synonyms

OMPdecase, OPRT, OPRTase, UMPS



Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, HEK-293T cells, COLO 320 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14830-1-AP targets UMPS in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UMPS fusion protein Ag6620
Full Name uridine monophosphate synthetase
Calculated Molecular Weight 52 kDa
Observed Molecular Weight 52 kDa, 45 kDa
GenBank Accession NumberBC000364
Gene Symbol UMPS
Gene ID (NCBI) 7372
RRIDAB_2212392
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UMPS, also named as OPRT and ODC, plays an important role in pyrimidine synthesis, converting orotic acid to uridine 5′ monophosphate. UMPS has four isoforms with MW 53 kDa, 43 kDa, 33 kDa and 23 kDa.

Protocols

Product Specific Protocols
WB protocol for UMPS antibody 14830-1-APDownload protocol
IHC protocol for UMPS antibody 14830-1-APDownload protocol
IP protocol for UMPS antibody 14830-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Metab

Macrophage-Released Pyrimidines Inhibit Gemcitabine Therapy in Pancreatic Cancer.

Authors - Christopher J Halbrook
  • KD Validated
humanIHC

Onco Targets Ther

Prognostic significance of thymidylate synthase in postoperative non-small cell lung cancer patients.

Authors - Hong-Yun Zhao
humanIHC

Medicine (Baltimore)

PMID: 25590835

Authors - Hong-Yun Zhao
humanIHC

ESMO Open

Immunohistochemical validation study of 15-gene biomarker panel predictive of benefit from adjuvant chemotherapy in resected non-small-cell lung cancer: analysis of JBR.10.

Authors - Stacy Grieve
humanWB

Nat Commun

Coordinative metabolism of glutamine carbon and nitrogen in proliferating cancer cells under hypoxia.

Authors - Yuanyuan Wang
humanWB

Cell Death Differ

Oxoglutarate dehydrogenase-like inhibits the progression of hepatocellular carcinoma by inducing DNA damage through non-canonical function

Authors - Xiang Jiang