UNC13A Polyclonal antibody

UNC13A Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 55053-1-AP

Synonyms

UNC13A/Munc13-1, Munc13-1, unc 13 homolog A (C. elegans), Protein unc-13 homolog A, Protein unc 13 homolog A

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Validation Data Gallery

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  WB analysis using 55053-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55053-1-AP (UNC13A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 55053-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55053-1-AP (UNC13A/Munc13-1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 55053-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 55053-1-AP (UNC13A/Munc13-1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 55053-1-AP

  IP result of anti-UNC13A (IP:55053-1-AP, 4ug; Detection:55053-1-AP 1:500) with mouse brain tissue lysate 5200ug.

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Tested Applications

• Positive WB detected in: mouse brain tissue, human brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Product Information

55053-1-AP targets UNC13A in WB, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: unc-13 homolog A (C. elegans)
• Calculated Molecular Weight: 193 kDa
• Observed Molecular Weight: 193-210 kDa
• GenBank Accession Number: NM_001080421
• Gene Symbol: UNC13A
• Gene ID (NCBI): 23025
• RRID: AB_10804173
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UNC13A, also named KIAA1032, belongs to the unc-13 family. It plays a role in vesicle maturation during exocytosis as a target of the diacylglycerol second messenger pathway. UNC13A is involved in neurotransmitter release by acting in synaptic vesicle priming prior to vesicle fusion and participates in the activity-dependent refilling of readily releasable vesicle pool (RRP). It is essential for synaptic vesicle maturation in most excitatory/glutamatergic but not inhibitory/GABA-mediated synapses. UNC13A is also involved in secretory granule priming in INS secretion. The antibody is specific to UNC13A.

Publications

Species	Application	Title
human	WB	Nature; TDP-43 represses cryptic exon inclusion in the FTD-ALS gene UNC13A.; Authors - X Rosa Ma
mouse	WB	Elife; Exophilin-8 assembles secretory granules for exocytosis in the actin cortex via interaction with RIM-BP2 and myosin-VIIa.; Authors - Fushun Fan
	CoIP	Cell Rep; Structural and Functional Analysis of the CAPS SNARE-Binding Domain Required for SNARE Complex Formation and Exocytosis.; Authors - Hao Zhou
human,mouse	WB,IF	Sci Adv; Phosphorylation of Doc2 by EphB2 modulates Munc13-mediated SNARE complex assembly and neurotransmitter release; Authors - Hong Zhang