UNC13A Recombinant monoclonal antibody, PBS Only

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  WB analysis using 83000-5-RR (same clone as 83000-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83000-5-RR (UNC13A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83000-5-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 83000-5-RR (same clone as 83000-5-PBS)

  IP result of anti-UNC13A (IP:83000-5-RR, 4ug; Detection:83000-5-RR 1:1000) with mouse brain tissue lysate 2100 ug. This data was developed using the same antibody clone with 83000-5-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 83000-5-RR (same clone as 83000-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83000-5-RR (UNC13A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83000-5-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 83000-5-RR (same clone as 83000-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83000-5-RR (UNC13A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83000-5-PBS in a different storage buffer formulation.

UNC13A Uni-rAb^® Recombinant Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 83000-5-PBS

Clone No.240017A6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Munc13-1, Protein unc-13 homolog A, Protein unc 13 homolog A, Munc13 1, KIAA1032

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Product Information

83000-5-PBS targets UNC13A in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: unc-13 homolog A (C. elegans)
• Calculated Molecular Weight: 193 kDa
• Observed Molecular Weight: 193-210 kDa
• GenBank Accession Number: NM_001080421
• Gene Symbol: UNC13A
• Gene ID (NCBI): 23025
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9UPW8
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

UNC13A, also named KIAA1032, belongs to the unc-13 family. It plays a role in vesicle maturation during exocytosis as a target of the diacylglycerol second messenger pathway. UNC13A is involved in neurotransmitter release by acting in synaptic vesicle priming prior to vesicle fusion and participates in the activity-dependent refilling of readily releasable vesicle pool (RRP). It is essential for synaptic vesicle maturation in most excitatory/glutamatergic but not inhibitory/GABA-mediated synapses. UNC13A is also involved in secretory granule priming in INS secretion. The antibody is specific to UNC13A.