UNC13A Recombinant antibody

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  IF Staining of rat brain using 83000-6-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using UNC13A antibody (83000-6-RR, Clone: 230469G2 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of rat brain using 83000-6-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using UNC13A antibody (83000-6-RR, Clone: 230469G2 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of SH-SY5Y using 83000-6-RR

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using UNC13A antibody (83000-6-RR, Clone: 230469G2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

UNC13A Uni-rAb^TM Recombinant Antibody for IF/ICC, IF-P, ELISA

Cat No. 83000-6-RR

Clone No.230469G2

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

IF/ICC, IF-P, ELISA

Synonyms

Munc13-1, Protein unc-13 homolog A, Protein unc 13 homolog A, Munc13 1, KIAA1032

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Tested Applications

• Positive IF-P detected in: rat brain tissue
• Positive IF/ICC detected in: SH-SY5Y cells

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83000-6-RR targets UNC13A in IF/ICC, IF-P, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: unc-13 homolog A (C. elegans)
• Calculated Molecular Weight: 193 kDa
• GenBank Accession Number: NM_001080421
• Gene Symbol: UNC13A
• Gene ID (NCBI): 23025
• RRID: AB_3670747
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9UPW8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UNC13A, also named KIAA1032, belongs to the unc-13 family. It plays a role in vesicle maturation during exocytosis as a target of the diacylglycerol second messenger pathway. UNC13A is involved in neurotransmitter release by acting in synaptic vesicle priming prior to vesicle fusion and participates in the activity-dependent refilling of readily releasable vesicle pool (RRP). It is essential for synaptic vesicle maturation in most excitatory/glutamatergic but not inhibitory/GABA-mediated synapses. UNC13A is also involved in secretory granule priming in INS secretion. The antibody is specific to UNC13A.