Tested Applications
| Positive WB detected in | Jurkat cells, K-562 cells, human placenta tissue |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human thymus tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16905-1-AP targets UNC13D/Munc13-4 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10519 Product name: Recombinant human UNC13D protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-327 aa of BC067084 Sequence: MATLLSHPQQRPPFLRQAIKIRRRRVRDLQDPPPQMAPEIQPPSHHFSPEQRALLYEDALYTVLHRLGHPEPNHVTEASELLRYLQEAFHVEPEEHQQTLQRVRELEKPIFCLKATVKQAKGILGKDVSGFSDPYCLLGIEQGVGVPGGSPGSRHRQKAVVRHTIPEEETHRTQVITQTLNPVWDETFILEFEDITNASFHLDMWDLDTVESVRQKLGELTDLHGLRRIFKEARKDKGQDDFLGNVVLRLQDLRCREDQWYPLEPRTETYPDRGQCHLQFQLIHKRRATSASRSQPSYTVHLHLLQQLVSHEVTQHEAGSTSWDGSL Predict reactive species |
| Full Name | unc-13 homolog D (C. elegans) |
| Calculated Molecular Weight | 1090 aa, 103 kDa |
| Observed Molecular Weight | 120 kDa |
| GenBank Accession Number | BC067084 |
| Gene Symbol | UNC13D |
| Gene ID (NCBI) | 201294 |
| RRID | AB_2212713 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q70J99 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for UNC13D/Munc13-4 antibody 16905-1-AP | Download protocol |
| IP protocol for UNC13D/Munc13-4 antibody 16905-1-AP | Download protocol |
| WB protocol for UNC13D/Munc13-4 antibody 16905-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-Munc13-4 (UNC13D) antibody, catalog number 16905-1-AP, has 7 total citations published in Blood, Molecular Cell, Journal of Experimental Medicine, Journal of Translational Medicine, Cell Reports, Frontiers in Immunology, and Molecular Biology of the Cell. These citations span research fields including primary immunodeficiency, hemophagocytic lymphohistiocytosis, pancreatic cancer cell migration, T-cell immune synapse architecture, endothelial exocytosis, and neuronal mRNA regulation.
| Species | Application | Title |
|---|---|---|
Blood Familial hemophagocytic lymphohistiocytosis type 3 (FHL3) caused by deep intronic mutation and inversion in UNC13D. | ||
Mol Cell Integrative omics indicate FMRP sequesters mRNA from translation and deadenylation in human neuronal cells | ||
J Exp Med Transcriptional regulation of Munc13-4 expression in cytotoxic lymphocytes is disrupted by an intronic mutation associated with a primary immunodeficiency. | ||
J Transl Med Recycling machinery of integrin coupled with focal adhesion turnover via RAB11-UNC13D-FAK axis for migration of pancreatic cancer cells | ||
Cell Rep Unveiling the molecular architecture of T cells and immune synapses with cryo-expansion microscopy. | ||
Front Immunol Alternative UNC13D Promoter Encodes a Functional Munc13-4 Isoform Predominantly Expressed in Lymphocytes and Platelets. |
