|Positive WB detected in||Jurkat cells, human placenta tissue, K-562 cells|
|Positive IP detected in||Jurkat cells|
|Positive IHC detected in||human thymus tissue, human kidney tissue, human lung tissue, human testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
16905-1-AP targets UNC13D/Munc13-4 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human samples.
|Host / Isotype||Rabbit / IgG|
|Immunogen||UNC13D/Munc13-4 fusion protein Ag10519|
|Full Name||unc-13 homolog D (C. elegans)|
|Calculated molecular weight||1090 aa, 103 kDa|
|Observed molecular weight||120 kDa|
|GenBank accession number||BC067084|
|Gene ID (NCBI)||201294|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Mol Biol Cell
A novel Munc13-4/S100A10/Annexin A2 complex promotes Weibel-Palade body exocytosis in endothelial cells.
Alternative UNC13D Promoter Encodes a Functional Munc13-4 Isoform Predominantly Expressed in Lymphocytes and Platelets.
Familial hemophagocytic lymphohistiocytosis type 3 (FHL3) caused by deep intronic mutation and inversion in UNC13D.
J Exp Med
Transcriptional regulation of Munc13-4 expression in cytotoxic lymphocytes is disrupted by an intronic mutation associated with a primary immunodeficiency.