Tested Applications
| Positive WB detected in | K-562 cells, mouse lung tissue, mouse ovary tissue, HeLa cells |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | human ovary cancer tissue, rat liver tissue, mouse stomach tissue, rat ovary tissue, rat stomach tissue, human stomach cancer tissue, mouse liver tissue, mouse ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
19564-1-AP targets UNC45A in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | unc-45 homolog A (C. elegans) |
| Calculated Molecular Weight | 103 kDa |
| Observed Molecular Weight | 100 kDa |
| GenBank Accession Number | NM_018671 |
| Gene Symbol | UNC45A |
| Gene ID (NCBI) | 55898 |
| RRID | AB_10644335 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H3U1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
UNC45A, also named as SMAP1, acts as co-chaperone for HSP90. UNC45A prevents the stimulation of HSP90AB1 ATPase activity by AHSA1. It is a positive factor in promoting PGR function in the cell. It is necessary for proper folding of myosin and necessary for normal cell proliferation, normal myotube formation and myosin accumulation during muscle cell development. UNC45A plays a role in erythropoiesis in stroma cells in the spleen. The antibody recognizes the C-term of UNC45A.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for UNC45A antibody 19564-1-AP | Download protocol |
| IP protocol for UNC45A antibody 19564-1-AP | Download protocol |
| WB protocol for UNC45A antibody 19564-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The UNC45A Polyclonal antibody (Cat# 19564-1-AP) has 3 total citations. These appear in Cells, FASEB J, and J Cell Sci. The cited research spans reproductive and nervous system biology (UNC-45A expression in mouse genital tract and microtubule-rich neural regions), intestinal epithelial barrier integrity and repair, and cytoskeletal biology (microtubule lattice disruption by UNC-45A). Applications used include IHC, IF, and WB across human, mouse, and rat species.
| Species | Application | Title |
|---|---|---|
Cells UNC-45A Is Highly Expressed in the Proliferative Cells of the Mouse Genital Tract and in the Microtubule-Rich Areas of the Mouse Nervous System. | ||
FASEB J A myosin chaperone, UNC-45A, is a novel regulator of intestinal epithelial barrier integrity and repair.
| ||
J Cell Sci UNC-45A breaks the microtubule lattice independently of its effects on non-muscle myosin II. |
Reviews
What customers say
The single review reports that the antibody performs well for Western blotting at a 1:1000 dilution. However, the user did not achieve satisfactory results when using it for Immunofluorescence at a 1:200 dilution in human liver cell lines. Overall, the product received a moderate rating, reflecting its strong WB performance but limited IF results under the tested conditions.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Kishor (Verified Customer) (10-17-2019) | This Ab works well for western blotting (1:1000) but I did not get satisfactory results for Immunofluorescence (1:200) in human liver cell lines.
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