Tested Applications
| Positive WB detected in | mouse skeletal muscle tissue, rat skeletal muscle tissue |
| Positive IHC detected in | mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21640-1-AP targets UNC45B in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag16294 Product name: Recombinant human UNC45B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC101063 Sequence: MAEVEAVQLKEEGNRHFQLQDYKAATNSYSQALKLTKDKALLATLYRNRAACGLKTESYIQAASDASRAIDINSSDIKALYRRCQALEHLGKLDQAFKDVQRCATLEPRNQNFQEMLRRLNTSIQEKLRVQFSTDSRVQKMFEILLDENSEADKREKAANNLIVLGREEAGAEKIFQNNGVALLLQLLDTKKPELVLAAVRTLSGMCSGHQARATVILHAVRIDRICSLMAVENEEMSLAVCNLLQAIIDSLSGEDKREHRGKEEALVLDTKKDLKQITSHLLDMLVSKKVSGQGRDQALNLLNKNVPRKDLAIHDNSRTIYVVDNGLRKILKVVGQVPDLPSCLPLTDN Predict reactive species |
| Full Name | unc-45 homolog B (C. elegans) |
| Calculated Molecular Weight | 931 aa, 104 kDa |
| Observed Molecular Weight | 95 kDa |
| GenBank Accession Number | BC101063 |
| Gene Symbol | UNC45B |
| Gene ID (NCBI) | 146862 |
| RRID | AB_10859787 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8IWX7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for UNC45B antibody 21640-1-AP | Download protocol |
| WB protocol for UNC45B antibody 21640-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The UNC45B Polyclonal antibody (Cat# 21640-1-AP) has a total of 2 citations. These appear in Journal of Cell Science (2025) and Molecular Biology of the Cell (2024). The research fields covered include Drosophila muscle development and function, as well as the role of the myosin chaperone UNC-45 in maintaining muscle sarcomere structure and function during adult aging in mice.
| Species | Application | Title |
|---|---|---|
J Cell Sci Distinct impacts of human co-chaperone UNC45 paralogs on Drosophila muscle development and function. | ||
Mol Biol Cell The myosin chaperone UNC-45 has an important role in maintaining the structure and function of muscle sarcomeres during adult aging |
Reviews
What customers say
The product has one verified review. A researcher using it for Western Blot on AC16 cells at a 1:1000 dilution (Lot 00083107) gave it a perfect 5-star rating and described the performance as "excellent." Overall, user feedback is positive, confirming reliable results in WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
PK (Verified Customer) (08-14-2024) | excellent
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