UNC5A-Specific Polyclonal antibody

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• UNC5A-Specific Polyclonal antibody (20239-1-AP)

UNC5A-Specific Polyclonal Antibody for WB, ELISA

Cat No. 20239-1-AP Copied!

Publications (2)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Synonyms

KIAA1976, Netrin receptor UNC5A, Protein unc-5 homolog 1, Protein unc-5 homolog A, UNC5A

• 

  WB analysis using 20239-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20239-1-AP (UNC5A-Specific antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse brain using 20239-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20239-1-AP (UNC5A-Specific antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: rat brain tissue, mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20239-1-AP targets UNC5A-Specific in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: unc-5 homolog A (C. elegans)
• Calculated Molecular Weight: 93 kDa
• Observed Molecular Weight: 103 kDa
• GenBank Accession Number: NM_133369
• Gene Symbol: UNC5A
• Gene ID (NCBI): 90249
• RRID: AB_10695050
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6ZN44
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UNC5A, also named as KIAA1976 and UNC5H1, belongs to the unc-5 family. It is a receptor for netrin required for axon guidance. It mediates axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding. Axon repulsion in growth cones may be caused by its association with DCC that may trigger signaling for repulsion. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand. The antibody is specific to UNC5A. It has no cross reaction to UNC5B, UNC5C and UNC5D.

Publications

What published studies show

The UNC5A-Specific Polyclonal antibody (Cat# 20239-1-AP) has 2 citations. One appears in Science Advances, studying the FLRT3-UNC5B checkpoint pathway in T cell-based cancer immunotherapies in humans. The other appears in Physiological Reports, investigating UNC5A as a key receptor in netrin-ligand-mediated fast-twitch myotube formation in mice using WB and IF. Research fields span cancer immunotherapy and neuromuscular development.

Automatically generated from the 2 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human		Sci Adv; The FLRT3-UNC5B checkpoint pathway inhibits T cell-based cancer immunotherapies; Authors - Kushal Prajapati
mouse	WB,IF	Physiol Rep; The uncoordinated-5 homologue A is a key receptor in netrin-ligand-mediated fast-twitch myotube formation in male mice.; Authors - Takahiro Maeno ; KD Validated