UNC5C-Specific Polyclonal antibody, PBS Only

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• UNC5C-Specific Polyclonal antibody, PBS Only (20240-1-PBS)

• 

  WB analysis using 20240-1-AP (same clone as 20240-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20240-1-AP (UNC5C-Specific antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20240-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 20240-1-AP (same clone as 20240-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20240-1-AP (UNC5C-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20240-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 20240-1-AP (same clone as 20240-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20240-1-AP (UNC5C-Specific antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20240-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 20240-1-AP (same clone as 20240-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20240-1-AP (UNC5C-Specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20240-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 20240-1-AP (same clone as 20240-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20240-1-AP (UNC5C-Specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20240-1-PBS in a different storage buffer formulation.

UNC5C-Specific Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 20240-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

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Product Information

20240-1-PBS targets UNC5C-Specific in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: unc-5 homolog C (C. elegans)
• Calculated Molecular Weight: 103 kDa
• Observed Molecular Weight: 50 kDa, 103 kDa
• GenBank Accession Number: NM_003728
• Gene Symbol: UNC5C
• Gene ID (NCBI): 8633
• RRID: AB_10695632
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O95185
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

UNC5C, also named as UNC5H3, belongs to the unc-5 family. It is a receptor for netrin required for axon guidance. It mediates axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding. Axon repulsion in growth cones may be caused by its association with DCC that may trigger signaling for repulsion. It also involved in corticospinal tract axon guidances independently of DCC. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand. The antibody is specific to UNC5C.