UPB1 Polyclonal antibody

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  WB analysis of mouse liver using 24603-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 24603-1-AP (UPB1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 24603-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 24603-1-AP (UPB1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 24603-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 24603-1-AP (UPB1 Antibody) at dilution of 1:50 (under 40x lens).

UPB1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 24603-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

Beta alanine synthase, Beta ureidopropionase, BUP 1, BUP1, UPB1, ureidopropionase, beta

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Tested Applications

• Positive WB detected in: mouse liver tissue
• Positive IHC detected in: human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

24603-1-AP targets UPB1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag18960

  Product name: Recombinant human UPB1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-384 aa of BC131703

  Sequence: MAGAEWKSLEECLEKHLPLPDLQEVKRVLYGKELRKLDLPREAFEAASREDFELQGYAFEAAEEQLRRPRIVHVGLVQNRIPLPANAPVAEQVSALHRRIKAIVEVAAMCGVNIICFQEAWTMPFAFCTREKLPWTEFAESAEDGPTTRFCQKLAKNHDMVVVSPILERDSEHGDVLWNTAVVISNSGAVLGKTRKNHIPRVGDFNESTYYMEGNLGHPVFQTQFGRIAVNICYGRHHPLNWLMYSINGAEIIFNPSATIGALSESLWPIEARNAAIANHCFTCAINRVGTEHFPNEFTSGDGKKAHQDFGYFYGSSYVAAPDSSRTPGLSRSRDGLLVAKLDLNLCQQVNDVWNFKMTGRYEMYARELAEAVKSNYSPTIVKE

• Full Name: ureidopropionase, beta
• Calculated Molecular Weight: 384 aa, 43 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC131703
• Gene Symbol: UPB1
• Gene ID (NCBI): 51733
• RRID: AB_2879634
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UBR1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BUP1(Beta-ureidopropionase) is also named as BUP1, Beta-alanine synthase, N-carbamoyl-beta-alanine amidohydrolase and belongs to the CN hydrolase family. It catalyzes the last step in the pyrimidine degradation pathway that it converts N-carbamyl-beta-aminoisobutyric acid and N-carbamyl-beta-alanine to, respectively, beta-aminoisobutyric acid and beta-alanine, ammonia and carbon dioxide. It is a protein with a calculated molecular mass of 43 kDa.