Tested Applications
| Positive WB detected in | HeLa cells, Raji cells, SH-SY5Y cells |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
23379-1-AP targets UPF1 in WB, IHC, IF/ICC, RIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag16195 Product name: Recombinant human UPF1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 769-1118 aa of BC039817 Sequence: EAANVEKITTKLLKAGAKPDQIGIITPYEGQRSYLVQYMQFSGSLHTKLYQEVEIASVDAFQGREKDFIILSCVRANEHQGIGFLNDPRRLNVALTRARYGVIIVGNPKALSKQPLWNHLLNYYKEQKVLVEGPLNNLRESLMQFSKPRKLVNTINPGARFMTTAMYDAREAIIPGSVYDRSSQGRPSSMYFQTHDQIGMISAGPSHVAAMNIPIPFNLVMPPMPPPGYFGQANGPAAGRGTPKGKTGRGGRQKNRFGLPGPSQTNLPNSQASQDVASQPFSQGALTQGYISMSQPSQMSQPGLSQPELSQDSYLGDEFKSQIDVALSQDSTYQGERAYQHGGVTGLSQY Predict reactive species |
| Full Name | UPF1 regulator of nonsense transcripts homolog (yeast) |
| Calculated Molecular Weight | 123 kDa |
| Observed Molecular Weight | 123-130 kDa |
| GenBank Accession Number | BC039817 |
| Gene Symbol | UPF1 |
| Gene ID (NCBI) | 5976 |
| RRID | AB_11232421 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q92900 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Up-Frameshift Suppressor 1 Homolog (UPF1) is the central factor in nonsense-mediated mRNA decay (NMD) and is also directly involved in telomere homeostasis, DNA replication, histone mRNA degradation and staufen-mediated mRNA decay (PMID: 29382845). It is a potential modulator of MALAT1 and that UPF1/MALAT1 pathway could be a therapeutic target for gastric cancer (PMID: 28942451). The molecular mass of UPF1 is 123-130 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for UPF1 antibody 23379-1-AP | Download protocol |
| IHC protocol for UPF1 antibody 23379-1-AP | Download protocol |
| WB protocol for UPF1 antibody 23379-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The UPF1 Polyclonal antibody (Cat# 23379-1-AP) has 16 citations across journals including Molecular Cell, EMBO Journal, Cell Reports, Science Advances, Cell Death & Disease, Journal of Experimental & Clinical Cancer Research, iScience, and others. Research fields span cancer biology (colorectal, glioblastoma, pancreatic, breast), neurodegeneration (ALS, spinocerebellar ataxia, C9orf72), mitochondrial dysfunction, RNA biology (nonsense-mediated decay, stress granules), immunology, and ribosome-associated protein dynamics. Applications are predominantly Western blot, with some IF and RIP.
| Species | Application | Title |
|---|---|---|
Mol Cell RAPIDASH: Tag-free enrichment of ribosome-associated proteins reveals composition dynamics in embryonic tissue, cancer cells, and macrophages | ||
J Exp Clin Cancer Res PTBP3 contributes to colorectal cancer growth and metastasis via translational activation of HIF-1α. | ||
Cell Death Dis PABPC1-induced stabilization of BDNF-AS inhibits malignant progression of glioblastoma cells through STAU1-mediated decay. | ||
Sci China Life Sci Compound heterozygous mutation of AFG3L2 causes autosomal recessive spinocerebellar ataxia through mitochondrial impairment and MICU1 mediated Ca2+ overload | ||
EMBO J ALS-associated RNA-binding proteins promote UNC13A transcription through REST downregulation | ||
Cell Mol Gastroenterol Hepatol Hyperglycemia Enhances Immunosuppression and Aerobic Glycolysis of Pancreatic Cancer Through Upregulating Bmi1-UPF1-HK2 Pathway. |
Reviews
What customers say
The single review for this product is a 5-star rating. The reviewer used the antibody at a 1:1000 dilution across Western Blot, Immunohistochemistry, and ChIP applications, describing the results as excellent with very visible, bright bands. Overall, the feedback highlights strong signal intensity and reliable performance across multiple techniques.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Mounika (Verified Customer) (12-25-2025) | Antibodies are excellent , very visible with bright bands!
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