UPF3A Polyclonal antibody

• Featured Product
• KD/KO Validated

UPF3A Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 17114-1-AP

Synonyms

hUpf3, HUPF3A, RENT3A, UPF3, UPF3A

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Validation Data Gallery

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  WB analysis using 17114-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17114-1-AP (UPF3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17114-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17114-1-AP (UPF3A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 17114-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 17114-1-AP (UPF3A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 17114-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17114-1-AP (UPF3A Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 17114-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17114-1-AP (UPF3A Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human heart using 17114-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 17114-1-AP (UPF3A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 17114-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 17114-1-AP (UPF3A antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 17114-1-AP

  Immunofluorescent analysis of HeLa cells using 17114-1-AP (UPF3A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, human testis tissue, Jurkat cells
• Positive IHC detected in: human testis tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

17114-1-AP targets UPF3A in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UPF3A fusion protein Ag10799
• Full Name: UPF3 regulator of nonsense transcripts homolog A (yeast)
• Calculated Molecular Weight: 443 aa, 51 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC023569
• Gene Symbol: UPF3A
• Gene ID (NCBI): 65110
• RRID: AB_10694141
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Cancer Manag Res; Functions and Clinical Significance of UPF3a Expression in Human Colorectal Cancer.; Authors - Xinmin Bao ; KD Validated
human	WB	Biochim Biophys Acta; Increase of a Group of PTC(+) Transcripts by Curcumin through Inhibition of the NMD Pathway.; Authors - Dairong Feng
human	IHC	J Gastroenterol Hepatol; Genetic compensation response could exist in colorectal cancer: UPF3A up-regulates the oncogenic homologue gene SRSF3 expression corresponding to SRSF6 to promote colorectal cancer metastasis; Authors - Weimin Xu