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UPF3A Polyclonal antibody
UPF3A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 17114-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, HeLa cells, human testis tissue, Jurkat cells |
Positive IHC detected in | human testis tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 2 publications below |
IHC | See 1 publications below |
Product Information
17114-1-AP targets UPF3A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | UPF3A fusion protein Ag10799 |
Full Name | UPF3 regulator of nonsense transcripts homolog A (yeast) |
Calculated Molecular Weight | 443 aa, 51 kDa |
Observed Molecular Weight | 55 kDa |
GenBank Accession Number | BC023569 |
Gene Symbol | UPF3A |
Gene ID (NCBI) | 65110 |
RRID | AB_10694141 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
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WB protocol for UPF3A antibody 17114-1-AP | Download protocol |
IHC protocol for UPF3A antibody 17114-1-AP | Download protocol |
IF protocol for UPF3A antibody 17114-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cancer Manag Res Functions and Clinical Significance of UPF3a Expression in Human Colorectal Cancer.
| ||
Biochim Biophys Acta Increase of a Group of PTC(+) Transcripts by Curcumin through Inhibition of the NMD Pathway. | ||
J Gastroenterol Hepatol Genetic compensation response could exist in colorectal cancer: UPF3A up-regulates the oncogenic homologue gene SRSF3 expression corresponding to SRSF6 to promote colorectal cancer metastasis |