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  • KD/KO Validated

UPF3A Polyclonal antibody

UPF3A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 17114-1-AP

Synonyms

Up-frameshift suppressor 3 homolog A, UPF3, RENT3A, HUPF3A, hUpf3



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, human testis tissue, Jurkat cells
Positive IHC detected inhuman testis tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17114-1-AP targets UPF3A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UPF3A fusion protein Ag10799
Full Name UPF3 regulator of nonsense transcripts homolog A (yeast)
Calculated Molecular Weight 443 aa, 51 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC023569
Gene Symbol UPF3A
Gene ID (NCBI) 65110
RRIDAB_10694141
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for UPF3A antibody 17114-1-APDownload protocol
IHC protocol for UPF3A antibody 17114-1-APDownload protocol
IF protocol for UPF3A antibody 17114-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Manag Res

Functions and Clinical Significance of UPF3a Expression in Human Colorectal Cancer.

Authors - Xinmin Bao
  • KD Validated
humanWB

Biochim Biophys Acta

Increase of a Group of PTC(+) Transcripts by Curcumin through Inhibition of the NMD Pathway.

Authors - Dairong Feng
humanIHC

J Gastroenterol Hepatol

Genetic compensation response could exist in colorectal cancer: UPF3A up-regulates the oncogenic homologue gene SRSF3 expression corresponding to SRSF6 to promote colorectal cancer metastasis

Authors - Weimin Xu