UPK1B Polyclonal antibody

UPK1B Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 26853-1-AP

Synonyms

Tetraspanin 20, Tspan 20, TSPAN20, UP1b, UPIB, UPK1, UPK1B, uroplakin 1B, Uroplakin Ib

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Validation Data Gallery

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  IHC staining of human urothelial carcinoma using 26853-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 26853-1-AP (UPK1B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 26853-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 26853-1-AP (UPK1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse bladder using 26853-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using 26853-1-AP (UPK1B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse bladder using 26853-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using 26853-1-AP (UPK1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: human urothelial carcinoma tissue, mouse bladder tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

26853-1-AP targets UPK1B in IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UPK1B fusion protein Ag25471
• Full Name: uroplakin 1B
• GenBank Accession Number: BC063568
• Gene Symbol: UPK1B
• Gene ID (NCBI): 7348
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Uroplakins are a group of urothelial differentiation-related membrane proteins. They are components of the asymmetric unit membrane (AUM), which forms the apical plaques of mammalian urothelium and is believed to play a role in strengthening the urothelial apical surface thus preventing the cells from rupturing during bladder distention (PMID: 8175808). Uroplakin-1B (UPK1B) belongs to the tetraspanin (TM4SF) family. UPK1B can promote the proliferation, invasion and metastasis of tumor cells and regulate the development and progression of tumors (PMID: 30229818).