Tested Applications
| Positive IP detected in | mouse kidney tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12964-1-AP targets UPP2 in IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3619 Product name: Recombinant human UPP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-317 aa of BC033529 Sequence: MASVIPASNRSMRSDRNTYVGKRFVHVKNPYLDLMDEDILYHLDLGTKTHNLPAMFGDVKFVCVGGSPNRMKAFALFMHKELGFEEAEEDIKDICAGTDRYCMYKTGPVLAISHGMGIPSISIMLHELIKLLHHARCCDVTIIRIGTSGGIGIAPGTVVITDIAVDSFFKPRFEQVILDNIVTRSTELDKELSEELFNCSKEIPNFPTLVGHTMCTYDFYEGQGRLDGALCSFSREKKLDYLKRAFKAGVRNIEMESTVFAAMCGLCGLKAAVVCVTLLDRLDCDQINLPHDVLVEYQQRPQLLISNFIRRRLGLCD Predict reactive species |
| Full Name | uridine phosphorylase 2 |
| Calculated Molecular Weight | 317 aa, 36 kDa |
| Observed Molecular Weight | 36 kDa |
| GenBank Accession Number | BC033529 |
| Gene Symbol | UPP2 |
| Gene ID (NCBI) | 151531 |
| RRID | AB_3741836 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95045 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Uridine phosphorylase (UPP) catalyzes the reversible phosphorolysis of uridine and chemically-related compounds to uracil and ribose-1-phosphate, implicating it in both pyrimidine salvage and regulation of uridine homeostasis .The enzyme is ubiquitously distributed amongst animals and bacteria, with most vertebrates possessing two versions of the phosphorylase, UPP1 and UPP2. While the two human homologues retain ~62% sequence identity, human UPP1 (hUPP1) was the first identified, cloned, and characterized, and consequently has been the focus of the majority of research . Not as much is known about the specific role of human UPP2 (hUPP2). Early studies showed that its tissue distribution was more limited than that of hUPP1, restricted predominantly to the kidneys, with some expression in the liver and spleen . (PMID: 21855639, PMID: 37473754)
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for UPP2 antibody 12964-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
