Tested Applications
| Positive WB detected in | MCF-7 cells, mouse heart tissue, mouse brain tissue, rat brain tissue, rat heart tissue |
| Positive IP detected in | mouse heart tissue |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18443-1-AP targets UQCRFS1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13315 Product name: Recombinant human UQCRFS1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-274 aa of BC010035 Sequence: MLSVAARSGPFAPVLSATSRGVAGALRPLVQATVPATPEQPVLDLKRPFLSRESLSGQAVRRPLVASVGLNVPASVCYSHTDIKVPDFSEYRRLEVLDSTKSSRESSEARKGFSYLVTGVTTVGVAYAAKNAVTQFVSSMSASADVLALAKIEIKLSDIPEGKNMAFKWRGKPLFVRHRTQKEIEQEAAVELSQLRDPQHDLDRVKKPEWVILIGVCTHLGCVPIANAGDFGGYYCPCHGSHYDASGRIRLGPAPLNLEVPTYEFTSDDMVIVG Predict reactive species |
| Full Name | ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 |
| Calculated Molecular Weight | 30 kDa |
| Observed Molecular Weight | 25 kDa |
| GenBank Accession Number | BC010035 |
| Gene Symbol | UQCRFS1 |
| Gene ID (NCBI) | 7386 |
| RRID | AB_10699875 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P47985 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The ubiquinol cytochrome c reductase UQCRFS1 is a key subunit of the cytochrome bc1 complex (complex III) of the mitochondrial respiratory chain. The UQCRFS1 gene encode a 30 kDa protein with a transit peptide and it appears to be involved in development of more aggressive phenotype of breast cancer(PMID:15047214). UQCRFS1 is also named as RISP, complex III subunit 5, cytochrome b-c1 complex subunit 5 and rieske iron-sulfur protein. This antibody is specific to UQCRFS1.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for UQCRFS1 antibody 18443-1-AP | Download protocol |
| IHC protocol for UQCRFS1 antibody 18443-1-AP | Download protocol |
| IP protocol for UQCRFS1 antibody 18443-1-AP | Download protocol |
| WB protocol for UQCRFS1 antibody 18443-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The UQCRFS1 polyclonal antibody (Cat# 18443-1-AP) has accumulated 40 citations published in high-impact journals including Cell, Nature Metabolism, Nature Cell Biology, Nature Chemical Biology, EMBO Journal, FASEB Journal, and PLoS ONE. The associated research fields encompass mitochondrial biology, oxidative phosphorylation, ferroptosis, cancer biology, neurodegeneration, cardiovascular disease, and iron-sulfur cluster metabolism.
| Species | Application | Title |
|---|---|---|
Cell Res NDUFAB1 confers cardio-protection by enhancing mitochondrial bioenergetics through coordination of respiratory complex and supercomplex assembly. | ||
Nat Metab Phenotypic profiling of solute carriers characterizes serine transport in cancer | ||
Nat Cell Biol Mitochondrial NADPH fuels mitochondrial fatty acid synthesis and lipoylation to power oxidative metabolism | ||
Nat Chem Biol Mitochondria-localized MBD2c facilitates mtDNA transcription and drug resistance |
Reviews
What customers say
The single review for this antibody is from Zee Chen (2020), who used it on adult mouse heart samples at a 1:10,000 dilution for Western Blot and reported nice results, giving it a 5-star rating.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
ZEE (Verified Customer) (07-13-2020) | WE TRY THIS ANTIBODY WITH MOUSE HEART SAMPLES, AND GOT NICE RESULTS.
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