Tested Applications
| Positive WB detected in | HeLa cells, Jurkat cells, HEK-293 cells, 4T1 cells, HSC-T6 cells, NIH/3T3 cells, pig brain tissue, rat brain tissue, mouse brain tissue |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68529-1-Ig targets UQCRFS1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.
| Tested Reactivity | human, mouse, rat, pig, rabbit |
| Cited Reactivity | mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13307 Product name: Recombinant human UQCRFS1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-274 aa of BC010035 Sequence: MLSVAARSGPFAPVLSATSRGVAGALRPLVQATVPATPEQPVLDLKRPFLSRESLSGQAVRRPLVASVGLNVPASVCYSHTDIKVPDFSEYRRLEVLDSTKSSRESSEARKGFSYLVTGVTTVGVAYAAKNAVTQFVSSMSASADVLALAKIEIKLSDIPEGKNMAFKWRGKPLFVRHRTQKEIEQEAAVELSQLRDPQHDLDRVKKPEWVILIGVCTHLGCVPIANAGDFGGYYCPCHGSHYDASGRIRLGPAPLNLEVPTYEFTSDDMVIVG Predict reactive species |
| Full Name | ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 |
| Calculated Molecular Weight | 30 kDa |
| Observed Molecular Weight | 25 kDa |
| GenBank Accession Number | BC010035 |
| Gene Symbol | UQCRFS1 |
| Gene ID (NCBI) | 7386 |
| RRID | AB_3085237 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P47985 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The ubiquinol cytochrome c reductase UQCRFS1 is a key subunit of the cytochrome bc1 complex (complex III) of the mitochondrial respiratory chain. The UQCRFS1 gene encode a 30 kDa protein with a transit peptide and it appears to be involved in development of more aggressive phenotype of breast cancer(PMID:15047214). UQCRFS1 is also named as RISP, complex III subunit 5, cytochrome b-c1 complex subunit 5 and rieske iron-sulfur protein.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for UQCRFS1 antibody 68529-1-Ig | Download protocol |
| WB protocol for UQCRFS1 antibody 68529-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The UQCRFS1 Monoclonal antibody (1F11C4), catalog number 68529-1-Ig, has a total of 2 citations. These citations appear in International Journal of Molecular Sciences and Journal of Proteomics. The associated research fields include skeletal muscle aging and mitochondrial DNA damage in cancerous conditions, as well as proteomics and phosphoproteomics analysis of acute pancreatitis. Both studies utilized the antibody for Western blot in mouse samples.
| Species | Application | Title |
|---|---|---|
Int J Mol Sci Cancerous Conditions Accelerate the Aging of Skeletal Muscle via Mitochondrial DNA Damage | ||
J Proteomics Proteomics and phosphoproteomics analysis of acute pancreatitis alleviated by forsythoside B |
