URG4 Monoclonal antibody

URG4 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2D5F12

Cat no : 67144-1-Ig

Synonyms

KIAA1507, up regulated gene 4, URG4, URGCP

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Validation Data Gallery

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  WB analysis using 67144-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67144-1-Ig (URG4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 67144-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67144-1-Ig (URG4 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 67144-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67144-1-Ig (URG4 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 67144-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67144-1-Ig (URG4 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 67144-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67144-1-Ig (URG4 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human nasopharyngeal carcinoma using 67144-1-Ig

  Immunohistochemical analysis of paraffin-embedded human nasopharyngeal carcinoma tissue slide using 67144-1-Ig (URG4 antibody) at dilution of 1:300 (under 40x lens).

• 

  IHC staining of human nasopharyngeal carcinoma using 67144-1-Ig

  Immunohistochemical analysis of paraffin-embedded human nasopharyngeal carcinoma tissue slide using 67144-1-Ig (URG4 antibody) at dilution of 1:300 (under 10x lens).

• 

  IHC staining of human liver cancer using 67144-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67144-1-Ig (URG4 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 67144-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67144-1-Ig (URG4 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 67144-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using URG4 antibody (67144-1-Ig, Clone: 2D5F12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: SGC-7901 cells, MKN-45 cells, HepG2 cells, HuH-7 cells, HSC-T6 cells, NIH/3T3 cells
• Positive IHC detected in: human breast cancer tissue, human liver cancer tissue, human nasopharyngeal carcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:20000
• Immunohistochemistry (IHC): IHC : 1:100-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67144-1-Ig targets URG4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: URG4 fusion protein Ag28635
• Full Name: up-regulated gene 4
• Calculated Molecular Weight: 922 aa, 104 kDa
• Observed Molecular Weight: 104 kDa
• GenBank Accession Number: BC018426
• Gene Symbol: URG4
• Gene ID (NCBI): 55665
• RRID: AB_2882443
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

URG4 (upregulated gene 4), also known as upregulator of cell proliferation (URGCP), is an oncogene which is involved in the development and progression of various tumors. URG4 is upregulated in hepatocellular carcinoma (HCC), ovarian cancer, and other types of cancer. Overexpression of URG4 not only results in tumour progression but also in metastasis and recurrence.