Tested Applications
| Positive WB detected in | K-562 cells, HepG2 cells, HEK-293 cells, rat kidney tissue, mouse liver tissue, rat liver tissue |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15547-1-AP targets UROD in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7905 Product name: Recombinant human UROD protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-367 aa of BC001778 Sequence: MEANGLGPQGFPELKNDTFLRAAWGEETDYTPVWCMRQAGRYLPEFRETRAAQDFFSTCRSPEACCELTLQPLRRFPLDAAIIFSDILVVPQALGMEVTMVPGKGPSFPEPLREEQDLERLRDPEVVASELGYVFQAITLTRQRLAGRVPLIGFAGAPWTLMTYMVEGGGSSTMAQAKRWLYQRPQASHQLLRILTDALVPYLVGQVVAGAQALQLFESHAGHLGPQLFNKFALPYIRDVAKQVKARLREAGLAPVPMIIFAKDGHFALEELAQAGYEVVGLDWTVAPKKARECVGKTVTLQVNLDPCALYASEEEIGQLVKQMLDDFGPHRYIANLGHGLYPDMDPEHVGAFVDAVHKHSRLLRQN Predict reactive species |
| Full Name | uroporphyrinogen decarboxylase |
| Calculated Molecular Weight | 41 kDa |
| Observed Molecular Weight | 41 kDa |
| GenBank Accession Number | BC001778 |
| Gene Symbol | UROD |
| Gene ID (NCBI) | 7389 |
| RRID | AB_2213813 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P06132 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for UROD antibody 15547-1-AP | Download protocol |
| IHC protocol for UROD antibody 15547-1-AP | Download protocol |
| IP protocol for UROD antibody 15547-1-AP | Download protocol |
| WB protocol for UROD antibody 15547-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The UROD Polyclonal antibody (Cat# 15547-1-AP) has 3 citations. These appear in Molecular Cell, Cell Chemical Biology, and Cell Molecular Gastroenterology and Hepatology. The research fields span heme transport and cellular iron availability, endogenous cereblon substrate detection and enrichment, and the histamine pathway in hepatic erythropoietic protoporphyria. All citations used the antibody for Western blot (WB) applications in human and/or mouse samples.
| Species | Application | Title |
|---|---|---|
Mol Cell Metabolic-scale gene activation screens identify SLCO2B1 as a heme transporter that enhances cellular iron availability. | ||
Cell Chem Biol A method for the detection and enrichment of endogenous cereblon substrates. | ||
Cell Mol Gastroenterol Hepatol The histamine pathway is a target to treat hepatic experimental erythropoietic protoporphyria |
