Tested Applications
| Positive WB detected in | HeLa cells, K-562 cells, Jurkat cells |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
22327-1-AP targets USF1 in WB, IHC, IF/ICC, ChIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag17789 Product name: Recombinant human USF1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-310 aa of BC035505 Sequence: MKGQQKTAETEEGTVQIQEGAVATGEDPTSVAIASIQSAATFPDPNVKYVFRTENGGQVMYRVIQVSEGQLDGQTEGTGAISGYPATQSMTQAVIQGAFTSDDAVDTEGTAAETHYTYFPSTAVGDGAGGTTSGSTAAVVTTQGSEALLGQATPPGTGQFFVMMSPQEVLQGGSQRSIAPRTHPYSPKSEAPRTTRDEKRRAQHNEVERRRRDKINNWIVQLSKIIPDCSMESTKSGQSKGGILSKACDYIQELRQSNHRLSEELQGLDQLQLDNDVLRQQVEDLKNKNLLLRAQLRHHGLEVVIKNDSN Predict reactive species |
| Full Name | upstream transcription factor 1 |
| Calculated Molecular Weight | 34 kDa |
| Observed Molecular Weight | 43 kDa |
| GenBank Accession Number | BC035505 |
| Gene Symbol | USF1 |
| Gene ID (NCBI) | 7391 |
| RRID | AB_2879073 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| UNIPROT ID | P22415 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Upstream stimulatory factor 1 (USF1),also name Class B basic helix-loop-helix protein 11. it is a major late transcription factor 1.USF1 is a member of the basic helix-loop-helix leucine zipper family, and can function as a cellular transcription factor. The encoded protein can activate transcription through pyrimidine-rich initiator (Inr) elements and E box motifs. USF1 has been linked to familial combined hyperlipidemia (FCHL). The USF1 exists two isoform and the calculated molecular weight of USF1 is a 32 kDa and 27 kDa. USF1 has acetylated form and phosphorylated form, so the molecular weight of USF1 can migrate to about 40 kDa
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for USF1 antibody 22327-1-AP | Download protocol |
| IHC protocol for USF1 antibody 22327-1-AP | Download protocol |
| WB protocol for USF1 antibody 22327-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The USF1 Polyclonal antibody (Cat# 22327-1-AP) has 11 citations published in journals including Cell Reports in Medicine, Genome Biology, International Immunopharmacology, Noncoding RNA Research, Cell Signaling, Functional & Integrative Genomics, Epigenetics, IUBMB Life, Digestive Diseases and Sciences, Journal of Oral Pathology and Medicine, and eLife. Research fields span oncology (gastric, oral, laryngeal, and renal cancers), cardiovascular disease (preeclampsia), sepsis-induced kidney injury, epigenetics, noncoding RNA biology, neurocognitive disorders, and leukemia.
| Species | Application | Title |
|---|---|---|
Cell Rep Med Nanoparticle-based serine replenishment rescues SP1-BNIP3 mitophagy and ameliorates preeclampsia in preclinical models. | ||
Genome Biol Acute depletion of CTCF rewires genome-wide chromatin accessibility.
| ||
Int Immunopharmacol CHMP2B exacerbates renal tubular injury in sepsis-induced acute kidney injury by enhancing autophagy blockade. | ||
Noncoding RNA Res USF1-activated hsa_circ_0076691 induces oxaliplatin resistance via facilitating FGF9 expression in miR-589-3p-dependent manners | ||
Cell Signal PUS1 facilitates cell migration in clear cell renal cell carcinoma through the promotion of mRNA pseudouridylation and the stabilization of the SMOX gene
| ||
Funct Integr Genomics USF1-mediated transcription of PARP1 contributes to sevoflurane-induced pyroptosis in perioperative neurocognitive disorders. |
