USF1 Polyclonal antibody

• Featured Product
• KD/KO Validated

USF1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 22327-1-AP

Synonyms

bHLHb11, FCHL, FCHL1, HYPLIP1, MLTF, MLTFI, UEF, Upstream stimulatory factor 1, USF, USF1

---

Validation Data Gallery

• 

  WB analysis using 22327-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22327-1-AP (USF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 22327-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22327-1-AP (USF1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 22327-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 22327-1-AP (USF1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach cancer using 22327-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 22327-1-AP (USF1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 22327-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 22327-1-AP (USF1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 22327-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 22327-1-AP (USF1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 22327-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 22327-1-AP (USF1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: HeLa cells, K-562 cells, Jurkat cells
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 3 publications below

Product Information

22327-1-AP targets USF1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: USF1 fusion protein Ag17789
• Full Name: upstream transcription factor 1
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC035505
• Gene Symbol: USF1
• Gene ID (NCBI): 7391
• RRID: AB_2879073
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Upstream stimulatory factor 1 (USF1),also name Class B basic helix-loop-helix protein 11. it is a major late transcription factor 1.USF1 is a member of the basic helix-loop-helix leucine zipper family, and can function as a cellular transcription factor. The encoded protein can activate transcription through pyrimidine-rich initiator (Inr) elements and E box motifs. USF1 has been linked to familial combined hyperlipidemia (FCHL). The USF1 exists two isoform and the calculated molecular weight of USF1 is a 32 kDa and 27 kDa. USF1 has acetylated form and phosphorylated form, so the molecular weight of USF1 can migrate to about 40 kDa

Publications

Species	Application	Title
human	WB	Genome Biol; Acute depletion of CTCF rewires genome-wide chromatin accessibility.; Authors - Beisi Xu ; KO Validated
human	WB	Elife; Functional interrogation of HOXA9 regulome in MLLr leukemia via reporter-based CRISPR/Cas9 screen.; Authors - Hao Zhang ; KO Validated
human,mouse	WB	J Oral Pathol Med; LncRNA LINC00152 promotes oral squamous cell carcinoma growth via enhancing Upstream Transcription Factor 1 mediated Mitochondrial Ribosomal Protein L52 transcription.; Authors - Xiuhe Zou ; KD Validated