Tested Applications
| Positive WB detected in | HEK-293 cells, PC-3 cells, mouse brain tissues, rat brain tissues |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | Human Colon cancer tissue, human kidney tissue, rat ovary tissue, mouse ovary tissue, rat small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11358-1-AP targets Harmonin in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1919 Product name: Recombinant human USH1C protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-392 aa of BC016057 Sequence: MDRKVAREFRHKVDFLIENDAEKDYLYDVLRMYHQTMDVAVLVGDLKLVINEPSRLPLFDAIRPLIPLKHQVEYDQLTPRRSRKLKEVRLDRLHPEGLGLSVRGGLEFGCGLFISHLIKGGQADSVGLQVGDEIVRINGYSISSCTHEEVINLIRTKKTVSIKVRHIGLIPVKSSPDEPLTWQYVDQFVSESGGVRGSLGSPGNRENKEKKVFISLVGSRGLGCSISSGPIQKPGIFISHVKPGSLSAEVGLEIGDQIVEVNGVDFSNLDHKEGRELFMTDRERLAEARQRELQRQELLMQKRLAMESNKILQEQQEMERQRRKEIAQKAAEENERYRKEMEQIVEEEEKFKKQWEEDWGSKEQLLLPKTITAEVHPVPLRKPKYDQGVEPE Predict reactive species |
| Full Name | Usher syndrome 1C (autosomal recessive, severe) |
| Calculated Molecular Weight | 552 aa, 62 kDa |
| Observed Molecular Weight | 68-73 kDa |
| GenBank Accession Number | BC016057 |
| Gene Symbol | USH1C/Harmonin |
| Gene ID (NCBI) | 10083 |
| RRID | AB_2214137 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y6N9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Harmonin, also known as USH1C, is a scaffold protein which interacts with other USH1 proteins (USH1B/myosin VIIa and USH1G/sans) to form complex and is implicated in proper development, morphogenesis, and structural integrity of the hair cell mechanoreceptor. Mutations of Harmonin cause Usher syndrome type 1 (congenital deafness and balance disorder, and early-onset sight loss).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Harmonin antibody 11358-1-AP | Download protocol |
| IHC protocol for Harmonin antibody 11358-1-AP | Download protocol |
| IP protocol for Harmonin antibody 11358-1-AP | Download protocol |
| WB protocol for Harmonin antibody 11358-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Harmonin (USH1C) polyclonal antibody (Cat# 11358-1-AP) has 3 total citations. These appear in International Journal of Molecular Sciences, Human Molecular Genetics, and Epigenomics. The associated research fields span prostate cancer (tumor immune environment and biochemical recurrence), Usher syndrome and olfaction, and DNA methylation changes during gallstone formation.
| Species | Application | Title |
|---|---|---|
Int J Mol Sci A Liquid-Liquid Phase Separation-Related Index Associate with Biochemical Recurrence and Tumor Immune Environment of Prostate Cancer Patients | ||
