Harmonin Polyclonal antibody

Harmonin Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11358-1-AP

Synonyms

AIE 75, AIE75, Antigen NY CO 38/NY CO 37, Harmonin, NY CO 37, NY CO 38, PDZ 45, PDZ 73, PDZ 73/NY CO 38, PDZ73, USH1C, Usher 1C



Tested Applications

Positive WB detected inHEK-293 cells, PC-3 cells, mouse brain tissues, rat brain tissues
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11358-1-AP targets Harmonin in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Harmonin fusion protein Ag1919
Full Name Usher syndrome 1C (autosomal recessive, severe)
Calculated Molecular Weight 552 aa, 62 kDa
Observed Molecular Weight 68-73 kDa
GenBank Accession NumberBC016057
Gene Symbol USH1C
Gene ID (NCBI) 10083
RRIDAB_2214137
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Harmonin, also known as USH1C, is a scaffold protein which interacts with other USH1 proteins (USH1B/myosin VIIa and USH1G/sans) to form complex and is implicated in proper development, morphogenesis, and structural integrity of the hair cell mechanoreceptor. Mutations of Harmonin cause Usher syndrome type 1 (congenital deafness and balance disorder, and early-onset sight loss).

Protocols

Product Specific Protocols
WB protocol for Harmonin antibody 11358-1-APDownload protocol
IHC protocol for Harmonin antibody 11358-1-APDownload protocol
IF protocol for Harmonin antibody 11358-1-APDownload protocol
IP protocol for Harmonin antibody 11358-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Hum Mol Genet

Impact of the Usher syndrome on olfaction.

Authors - Fabian Jansen
humanIHC

Int J Mol Sci

A Liquid-Liquid Phase Separation-Related Index Associate with Biochemical Recurrence and Tumor Immune Environment of Prostate Cancer Patients

Authors - Qi You