USH1G Polyclonal antibody

USH1G Polyclonal Antibody for IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Mouse

Applications

IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21936-1-AP

Synonyms

ANKS4A, SANS, USH1G, Usher syndrome type 1G protein

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Validation Data Gallery

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  IP experiment of NIH/3T3 using 21936-1-AP

  IP result of anti-USH1G (IP:21936-1-AP, 4ug; Detection:21936-1-AP 1:500) with NIH/3T3 cells lysate 1200ug.

• 

  IHC staining of mouse testis using 21936-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21936-1-AP (USH1G antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 21936-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21936-1-AP (USH1G antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 21936-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21936-1-AP (USH1G antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 21936-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21936-1-AP (USH1G antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IP detected in: NIH/3T3 cells
• Positive IHC detected in: mouse testis tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21936-1-AP is USH1G Fusion Protein expressed in E. coli.

• Tested Reactivity: Mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: USH1G fusion protein Ag16571
• Full Name: Usher syndrome 1G (autosomal recessive)
• Calculated Molecular Weight: 461 aa, 51 kDa
• Observed Molecular Weight: 52-55 kDa
• GenBank Accession Number: BC101099
• Gene Symbol: USH1G
• Gene ID (NCBI): 124590
• RRID: AB_2878950
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.