USHBP1 Polyclonal antibody

USHBP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 17226-1-AP

Synonyms

MCC2, MCC 2, Harmonin-binding protein USHBP1, AIEBP, AIE-75-binding protein

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Validation Data Gallery

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  WB analysis of A549 using 17226-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 17226-1-AP (USHBP1 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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  WB analysis of mouse heart using 17226-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 17226-1-AP (USHBP1 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

• 

  WB analysis of COLO 320 using 17226-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 17226-1-AP (USHBP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 17226-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 17226-1-AP (USHBP1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human small intestine using 17226-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 17226-1-AP (USHBP1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 17226-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 17226-1-AP (USHBP1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human small intestine using 17226-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 17226-1-AP (USHBP1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of A549 using 17226-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed A549 cells using 17226-1-AP (USHBP1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells, COLO 320 cells, mouse heart tissue
• Positive IHC detected in: human heart tissue, human small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17226-1-AP targets USHBP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: USHBP1 fusion protein Ag11064
• Full Name: Usher syndrome 1C binding protein 1
• Calculated Molecular Weight: 703 aa, 76 kDa
• Observed Molecular Weight: 90-100 kDa
• GenBank Accession Number: BC027910
• Gene Symbol: USHBP1
• Gene ID (NCBI): 83878
• RRID: AB_2878364
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

USHBP1, also named as Mutated in colon cancer protein 2 or AIE-75-binding protein, is a 703 amino acid protein, which belongs to the MCC family. Highest level of expression is in heart, and moderate to low expression in skeletal muscle, kidney, liver, small intestine, placenta and lung.